IHC (Immunohistochemisry)
(HGF/Hepatocyte Growth Factor Antibody-Anti-HGF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
Rabbit anti-Rat, Human HGF/Hepatocyte Growth Factor Polyclonal Antibody | anti-HGF antibody
HGF/Hepatocyte Growth Factor Rabbit anti-Human Polyclonal (aa216-265) Antibody
Reactivity
Rat, Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunoaffinity purified
Synonyms
HGF/Hepatocyte Growth Factor, Antibody; HGF/Hepatocyte Growth Factor Rabbit anti-Human Polyclonal (aa216-265) Antibody; PathPlus HGF/Hepatocyte Growth Factor Antibody (aa216-265); HGF; DFNB39; Hepatocyte growth factor; Hepatopoeitin-A; Hepapoietin A; Hepatopoietin-A; Scatter factor); SF; F-TCF; HGFB; HPTA; Scatter factor; anti-HGF antibody
Host
Rabbit
Reactivity
Rat, Human
Clonality
Polyclonal
Specificity
Human HGF
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.09% sodium azide, 2% sucrose
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-HGF antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Target
Human HGF/Hepatocyte Growth Factor
Immunogen
Synthetic peptide located between aa216-265 of human HGF (P14210, NP_000592). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%); Bat (92%); Turkey, Zebra finch, Chicken (85%).
Conjugation
Unconjugated
Epitope
aa216-265
Family
Protease
Subfamily
Serine S1
Preparation and Storage
Short term: Store at 2-8 degree C. Long term: Aliquot and store at -20 degree C. Avoid freeze/thaw cycles.
IHC (Immunohistochemisry)
(HGF/Hepatocyte Growth Factor Antibody-Anti-HGF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohistochemisry)
(HGF/Hepatocyte Growth Factor Antibody-Anti-HGF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohiostchemistry)
(HGF/Hepatocyte Growth Factor Antibody-Anti-HGF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohiostchemistry)
(HGF/Hepatocyte Growth Factor Antibody-Anti-HGF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
WB (Western Blot)
(HGF/Hepatocyte Growth Factor Antibody-HGF antibody Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
WB (Western Blot)
(HGF/Hepatocyte Growth Factor Antibody-HGF antibody Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
Related Product Information for anti-HGF antibody
Hepatocyte Growth Factor antibody is an unconjugated rabbit polyclonal antibody to Hepatocyte Growth Factor (HGF)(aa216-265) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
HGF (Hepatocyte growth factor, scatter factor) is a protein secreted by mesenchymal cells that functions in paracrine cell motility, growth and morphogenesis, as well as embryogenesis, organ regeneration and wound healing. It targets epithelial cells, endothelial cells, oligodendrocyte precursor cells, hameopoietic progenitor cells and T cells to induce migration as well as proliferative and angiogenic processes. Levels of expression of HGF are positively correlated with number of neocortical GABAergic interneurons and decreased HGF levels are associated with susceptibility to seizures -- increasing HGF levels has been shown to help lower susceptibility. HGF can activate Schwann cells to promote peripheral nerve regeneration. It is also thought to be involved in atherogenesis and is found in atherosclerotic plaques. In immunohistochemistry, HGF has moderate extracellular and cytoplasmic positivity in most tissues throughout the body.
HGF (Hepatocyte growth factor, scatter factor) is a protein secreted by mesenchymal cells that functions in paracrine cell motility, growth and morphogenesis, as well as embryogenesis, organ regeneration and wound healing. It targets epithelial cells, endothelial cells, oligodendrocyte precursor cells, hameopoietic progenitor cells and T cells to induce migration as well as proliferative and angiogenic processes. Levels of expression of HGF are positively correlated with number of neocortical GABAergic interneurons and decreased HGF levels are associated with susceptibility to seizures -- increasing HGF levels has been shown to help lower susceptibility. HGF can activate Schwann cells to promote peripheral nerve regeneration. It is also thought to be involved in atherogenesis and is found in atherosclerotic plaques. In immunohistochemistry, HGF has moderate extracellular and cytoplasmic positivity in most tissues throughout the body.
References
Biomed Res Int. 2013;2013:470418, PMID: 24490163; EXS. 1993;65:225-49, PMID: 8422545; Exp Cell Res. 2001 Jul 15;267(2):258-66, PMID: 11426944; Atherosclerosis. 2002 Sep;164(1):79-87; Atherosclerosis. 2002 Sep;164(1):79-87, PMID: 12119196; Scientific Reports. 2018. 8 (8316), DOI: 10.1038/s41598-018-26704-x; J Neurosci Res. 2002 Sep 1;69(5):597-606, PMID: 12210825;
NCBI and Uniprot Product Information
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Product Notes
The HGF hgf (Catalog #AAA162513) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HGF/Hepatocyte Growth Factor Rabbit anti-Human Polyclonal (aa216-265) Antibody reacts with Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's HGF/Hepatocyte Growth Factor can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the HGF hgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HGF/Hepatocyte Growth Factor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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