WB (Western Blot)
(HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)
Rabbit anti-Human HHLA2 Polyclonal Antibody | anti-HHLA2 antibody
HHLA2 Antibody (N-term)
WB~~1:1000
FC~~1:10~50
WB (Western Blot)
(HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)
WB (Western Blot)
(HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)
FCM (Flow Cytometry)
(HHLA2 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(HHLA2 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(HHLA2 Antibody (N-term) (AAA28722)immunohistochemistry analysis in formalin fixed and paraffin embedded human coloncarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the HHLA2Antibody (N-term) for immunohistochemistry.Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(HHLA2 Antibody (N-term) (AAA28722)immunohistochemistry analysis in formalin fixed and paraffin embedded human coloncarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the HHLA2Antibody (N-term) for immunohistochemistry.Clinical relevance has not been evaluated.)
WB (Western Blot)
(All lanes : Anti-HHLA2 Antibody (N-term) at1:2000 dilution Lane 1: human pancreas lysateLane 2: MDA-MB-453 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 47 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-HHLA2 Antibody (N-term) at1:2000 dilution Lane 1: human pancreas lysateLane 2: MDA-MB-453 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 47 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-HHLA2 Antibody (N-term) at1:2000 dilution Lane 1: MDA-MB-453 whole celllysate Lane 2: Human lung lysate Lane 3:Human testis lysate Lysates/proteins at 20 µgper lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-HHLA2 Antibody (N-term) at1:2000 dilution Lane 1: MDA-MB-453 whole celllysate Lane 2: Human lung lysate Lane 3:Human testis lysate Lysates/proteins at 20 µgper lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
IHC (Immunohistochemistry)
(AAA28722 staining HHLA2 in human kidney tissue sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA28722 staining HHLA2 in human kidney tissue sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
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Product Notes
The HHLA2 hhla2 (Catalog #AAA28722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 58-87. The HHLA2 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HHLA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). IHC-P~~1:50~100 WB~~1:1000 FC~~1:10~50. Researchers should empirically determine the suitability of the HHLA2 hhla2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HHLA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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