IF (Immunofluorescence)
(Immunofluorescence staining of HIGD2A-FP06572 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human HIGD2A-FP06572 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human HIGD2A Polyclonal Antibody | anti-HIGD2A antibody
Anti-HIGD2A Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
HIGD2A, Antibody; Anti-HIGD2A Antibody, Rabbit Polyclonal; HIGD2A Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-HIG2A Antibody; Anti-MGC2198 Antibody; Anti-RCF1b Antibody; anti-HIGD2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human HIGD2A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-HIGD2A antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E coli-derived Human HIGD2A fragment
Preparation
Produced in rabbits immunized with E coli-derived Human HIGD2A fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
IF (Immunofluorescence)
(Immunofluorescence staining of HIGD2A-FP06572 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human HIGD2A-FP06572 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of HIGD2A-FP06572 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human HIGD2A-FP06572 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human HIGD2A in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human HIGD2A in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-HIGD2A rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse kidney tissue lysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:11 kDaObserved band size:14 kDa)
WB (Western Blot)
(Anti-HIGD2A rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse kidney tissue lysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:11 kDaObserved band size:14 kDa)
Related Product Information for anti-HIGD2A antibody
The expression of HIGD2A is dependent on oxygen levels, glucose concentration, and cell cycle progression. This gene encodes for protein HIG2A, found in mitochondria and the nucleus, promoting cell survival in hypoxic conditions. The encoded HIGD2A protein is an inner mitochondrial membrane protein and is a functional ortholog of the yeast respiratory supercomplex factor 1 (Rcf1). In mouse, the orthologous protein enhances cell survival under conditions of hypoxia. The genomic location of HIGD2A is in chromosome 5q35.2, where several chromosomal abnormalities are related to numerous cancers. Depletion of HIGD2A selectively impairs the viability of colon adenocarcinoma cells (DLD1), which are Ras mutant cells, suggesting a role of HIG2A in cell cycle regulation and a potential target in cancer therapy.
References
Salaza. C. e. al. (2020. Biosyste. analysi. o. th. hypoxi. inducibl. domai. famil. membe. 2a. Implication. i. cance. biology. Gene. (Basel. 1. (2). 206.
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Product Notes
The HIGD2A (Catalog #AAA259452) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HIGD2A Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIGD2A can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HIGD2A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIGD2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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