WB (Western Blot)
(Western blot analysis of extracts from PC-3 cells, using Hikeshi Antibody. The lane on the left was treated with blocking peptide.)
Rabbit Hikeshi Polyclonal Antibody | anti-HIKESHI antibody
Hikeshi Antibody
Gene Names
HIKESHI; HLD13; L7RN6; OPI10; HSPC138; HSPC179; C11orf73
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Hikeshi, Antibody; Hikeshi Antibody; anti-HIKESHI antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Hikeshi Antibody detects endogenous levels of Hikeshi.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
197
Applicable Applications for anti-HIKESHI antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human Hikeshi.
Tissue Specificity
Following heat-shock treatment.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus; Cytosol
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from PC-3 cells, using Hikeshi Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from PC-3 cells, using Hikeshi Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela, using Hikeshi Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela, using Hikeshi Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-HIKESHI antibody
Description: Acts as a specific nuclear import carrier for HSP70 proteins following heat-shock stress: acts by mediating the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus. HSP70 proteins import is required to protect cells from heat shock damages. Does not translocate ADP-bound HSP70 proteins into the nucleus.
Subunit Structure: Forms an asymmetric homodimer; required for binding and nuclear import of HSP70 proteins (PubMed:25760597). Interacts with ATP-bound HSP70 proteins (PubMed:22541429). Interacts with NUP62 and NUP153 (via F-X-F-G repeats) (PubMed:22541429). Interacts with HSPA8 (PubMed:25760597).
Similarity: Belongs to the OPI10 family.
Subunit Structure: Forms an asymmetric homodimer; required for binding and nuclear import of HSP70 proteins (PubMed:25760597). Interacts with ATP-bound HSP70 proteins (PubMed:22541429). Interacts with NUP62 and NUP153 (via F-X-F-G repeats) (PubMed:22541429). Interacts with HSPA8 (PubMed:25760597).
Similarity: Belongs to the OPI10 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 22 kDa
Predicted: 22 kDa
Predicted: 22 kDa
NCBI Official Full Name
protein Hikeshi isoform 1
NCBI Official Synonym Full Names
heat shock protein nuclear import factor hikeshi
NCBI Official Symbol
HIKESHI
NCBI Official Synonym Symbols
HLD13; L7RN6; OPI10; HSPC138; HSPC179; C11orf73
NCBI Protein Information
protein Hikeshi
UniProt Protein Name
Protein Hikeshi
UniProt Gene Name
C11orf73
UniProt Entry Name
HIKES_HUMAN
Similar Products
Product Notes
The HIKESHI c11orf73 (Catalog #AAA328670) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hikeshi Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Hikeshi can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the HIKESHI c11orf73 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hikeshi, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.