HINT1 Polyclonal Antibody | anti-HINT1 antibody

Anti-HINT1 Antibody

Cat. Num. AAA46429

• Gene Names: HINT1; HINT; NMAN; PKCI-1; PRKCNH1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: HINT1, Antibody; Anti-HINT1 Antibody; Histidine triad nucleotide-binding protein 1; HINT 1; HINT1; HINT1_HUMAN; Histidine triad nucleotide binding protein 1; PKCI 1; PKCI-1; PKCI1; PRKCNH1; Protein kinase C inhibitor 1; Protein kinase C interacting protein 1; Protein kinase C-interacting protein 1; histidine triad nucleotide binding protein 1; anti-HINT1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 126

• Applicable Applications for anti-HINT1 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HINT1 (59-92aa HISQISVAEDDDESLLGHLMIVGKKCAADLGLNK), different from the related mouse and rat sequences by three amino acids.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(Anti- HINT1 Picoband antibody, AAA46429, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

IHC (Immunohistochemisry)

(Anti- HINT1 Picoband antibody, AAA46429, IHC(P)IHC(P): Rat Kidney Tissue)

IHC (Immunohiostchemistry)

(Anti- HINT1 Picoband antibody, AAA46429, IHC(P)IHC(P): Mouse Intestine Tissue)

WB (Western Blot)

(Anti- HINT1 Picoband antibody, AAA46429, Western blottingAll lanes: Anti HINT1 (AAA46429) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Thymus Tissue Lysate at 50ugLane 3: Rat Testis Tissue Lysate at 50ugLane 4: Rat Skeletal Muscle Tissue Lysate at 50ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 14KDObserved bind size: 14KD)

Related Product Information for anti-HINT1 antibody

Description: Rabbit IgG polyclonal antibody for Histidine triad nucleotide-binding protein 1(HINT1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Histidine triad nucleotide-binding protein 1 is a protein that in humans is encoded by the HINT1 gene. It is a haploinsufficient tumor suppressor gene that inhibits the Wnt/beta-catenin pathway in colon cancer cells and Microphthalmia-associated transcription factor (MITF) activity in human mast cells. The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. Several transcript variants, but only one of them protein-coding, have been found for this gene.

References

1. "Entrez Gene: HINT1 histidine triad nucleotide binding protein 1". 2. Brzoska PM, Chen H, Levin NA, Kuo WL, Collins C, Fu KK, Gray JW, Christman MF (Feb 1997). "Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)". Genomics 36(1): 151-6. 3. Wang L, Li H, Zhang Y, Santella RM, Weinstein IB (Apr 2009). "HINT1 inhibits beta-catenin/TCF4, USF2 and NFkappaB activity in human hepatoma cells". Int J Cancer 124 (7): 1526-34.

NCBI and Uniprot Product Information

• NCBI GI #: 4885413
• NCBI GeneID: 3094
• NCBI Accession #: NP_005331.1
• NCBI GenBank Nucleotide #: NM_005340.6
• UniProt Accession #: P49773; Q9H5W8
• Molecular Weight: 13,802 Da
• NCBI Official Full Name: histidine triad nucleotide-binding protein 1
• NCBI Official Synonym Full Names: histidine triad nucleotide binding protein 1
• NCBI Official Symbol: HINT1
• NCBI Official Synonym Symbols: HINT; NMAN; PKCI-1; PRKCNH1
• NCBI Protein Information: histidine triad nucleotide-binding protein 1
• UniProt Protein Name: Histidine triad nucleotide-binding protein 1
• UniProt Gene Name: HINT1
• UniProt Synonym Gene Names: HINT; PKCI1; PRKCNH1; PKCI-1
• UniProt Entry Name: HINT1_HUMAN