WB (Western Blot)
(All lanes : Anti-HIST1H2BM Antibody (N-term) at 1:2000 dilutionLane 1: PC-3 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit HIST1H2BM Polyclonal Antibody | anti-HIST1H2BM antibody
HIST1H2BM Antibody (N-term)
Gene Names
H2BC14; H2B/e; H2BFE; HIST1H2BM; dJ160A22.3
Reactivity
HumanPredicted: Mouse
Applications
ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
HIST1H2BM, Antibody; HIST1H2BM Antibody (N-term); Histone H2B type 1-M; Histone H2Be; H2B/e; HIST1H2BM; H2BFE; anti-HIST1H2BM antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This HIST1H2BM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 10-44 amino acids from the N-terminal region of human HIST1H2BM.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
126
Applicable Applications for anti-HIST1H2BM antibody
ELISA, WB (Western Blot)
Cellular Location
Nucleus. Chromosome.
Antigen Region
10-44
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-HIST1H2BM Antibody (N-term) at 1:2000 dilutionLane 1: PC-3 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-HIST1H2BM Antibody (N-term) at 1:2000 dilutionLane 1: PC-3 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Western blot analysis of lysates from human skeletal muscle tissue and PC-3, K562 cell line (from left to right), using HIST1H2BM Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of lysates from human skeletal muscle tissue and PC-3, K562 cell line (from left to right), using HIST1H2BM Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)
Related Product Information for anti-HIST1H2BM antibody
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
References
Albig W.,et al.Hum. Genet. 101:284-294(1997). Marzluff W.F.,et al.Genomics 80:487-498(2002). Mungall A.J.,et al.Nature 425:805-811(2003). Lubec G.,et al.Submitted (MAR-2007) to UniProtKB. Cheung W.L.,et al.Cell 113:507-517(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
H: 14kDa; M: 14 kDa
NCBI Official Full Name
Histone H2B type 1-M
NCBI Official Synonym Full Names
H2B clustered histone 14
NCBI Official Symbol
H2BC14
NCBI Official Synonym Symbols
H2B/e; H2BFE; HIST1H2BM; dJ160A22.3
NCBI Protein Information
histone H2B type 1-M
UniProt Protein Name
Histone H2B type 1-M
UniProt Gene Name
HIST1H2BM
UniProt Synonym Gene Names
H2BFE; H2B/e
UniProt Entry Name
H2B1M_HUMAN
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Product Notes
The HIST1H2BM hist1h2bm (Catalog #AAA290934) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIST1H2BM Antibody (N-term) reacts with Human Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HIST1H2BM can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HIST1H2BM hist1h2bm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIST1H2BM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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