IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Histidine Decarboxylase/HDC using anti-Histidine Decarboxylase/HDC antibody (AAA126184).Histidine Decarboxylase/HDC was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Histidine Decarboxylase/HDC Antibody (AAA126184) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
Rabbit anti-Human, Mouse Histidine decarboxylase/HDC Polyclonal Antibody | anti-HDC antibody
Anti-Histidine decarboxylase/HDC Antibody Picoband
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Histidine decarboxylase/HDC, Antibody; Anti-Histidine decarboxylase/HDC Antibody Picoband; anti-HDC antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Natural and recombinant Human CD55
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-HDC antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Assay Type
Sandwich
Samples
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<50 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Histidine Decarboxylase/HDC using anti-Histidine Decarboxylase/HDC antibody (AAA126184).Histidine Decarboxylase/HDC was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Histidine Decarboxylase/HDC Antibody (AAA126184) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Histidine Decarboxylase/HDC using anti-Histidine Decarboxylase/HDC antibody (AAA126184).Histidine Decarboxylase/HDC was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Histidine Decarboxylase/HDC Antibody (AAA126184) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
WB (Western Blot)
(Figure 1. Western blot analysis of Histidine Decarboxylase/HDC using anti-Histidine Decarboxylase/HDC antibody (AAA126184).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEL whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human Hacat whole cell lysates,Lane 4: human HL-60 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histidine Decarboxylase/HDC antigen affinity purified polyclonal antibody (#AAA126184) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Histidine Decarboxylase/HDC at approximately 74 kDa. The expected band size for Histidine Decarboxylase/HDC is at 74 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of Histidine Decarboxylase/HDC using anti-Histidine Decarboxylase/HDC antibody (AAA126184).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEL whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human Hacat whole cell lysates,Lane 4: human HL-60 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histidine Decarboxylase/HDC antigen affinity purified polyclonal antibody (#AAA126184) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Histidine Decarboxylase/HDC at approximately 74 kDa. The expected band size for Histidine Decarboxylase/HDC is at 74 kDa.)
Related Product Information for anti-HDC antibody
Background: Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. It is mapped to 1q32.2. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
Principle of the Assay: The Quick Picokine™ Human CD55 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD55 in cell culture supernatants, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human CD55 with immunogen: Expression system for standard: NS0; Immunogen sequence: D35-S353. To measure Human CD55, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CD55 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD55 in the sample.
Principle of the Assay: The Quick Picokine™ Human CD55 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD55 in cell culture supernatants, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human CD55 with immunogen: Expression system for standard: NS0; Immunogen sequence: D35-S353. To measure Human CD55, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CD55 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD55 in the sample.
Product Categories/Family for anti-HDC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,059 Da
NCBI Official Full Name
histidine decarboxylase
NCBI Official Synonym Full Names
histidine decarboxylase
NCBI Official Symbol
HDC
NCBI Protein Information
histidine decarboxylase
UniProt Protein Name
Histidine decarboxylase
UniProt Gene Name
HDC
UniProt Synonym Gene Names
HDC
UniProt Entry Name
DCHS_HUMAN
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Product Notes
The HDC hdc (Catalog #AAA126184) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Histidine decarboxylase/HDC Antibody Picoband reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histidine decarboxylase/HDC can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HDC hdc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histidine decarboxylase/HDC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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