Application Data
(ChIP of 2ug with 1ug MCF7 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.)
Goat Histone Deacetylase 1 Polyclonal Antibody | anti-HDAC1 antibody
Goat anti-Histone Deacetylase 1 Antibody
Gene Names
HDAC1; HD1; RPD3; GON-10; RPD3L1
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Histone Deacetylase 1, Antibody; Goat anti-Histone Deacetylase 1 Antibody; Goat Anti-Hdac1 (aa385-396) Antibody; HDAC1; histone deacetylase 1; DKFZp686H12203; GON-10; HD1; RPD3; RPD3L1; reduced potassium dependency, yeast homolog-like 1; HDAC1 antibody; histone deacetylase 1 antibody; DKFZp686H12203 antibody; GON-10 antibody; HD1 antibody; RPD3 antibody; RPD3L1 antibody; reduced potassium dependency; yeast homolog-like 1 antibody; Histone Deacetylase 1; anti-HDAC1 antibody
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KPEAKGVKEEVK
Sequence Length
482
Applicable Applications for anti-HDAC1 antibody
ChIP (Chromatin immunoprecipitation), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-KPEAKGVKEEVK, from the C Terminus of the protein sequence according to NP_004955.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Application Data
(ChIP of 2ug with 1ug MCF7 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.)
Application Data
(ChIP of 2ug with 1ug MCF7 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.)
WB (Western Blot)
((0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,103 Da
NCBI Official Full Name
histone deacetylase 1
NCBI Official Synonym Full Names
histone deacetylase 1
NCBI Official Symbol
HDAC1
NCBI Official Synonym Symbols
HD1; RPD3; GON-10; RPD3L1
NCBI Protein Information
histone deacetylase 1
UniProt Protein Name
Histone deacetylase 1
UniProt Gene Name
HDAC1
UniProt Synonym Gene Names
RPD3L1; HD1
UniProt Entry Name
HDAC1_HUMAN
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Product Notes
The HDAC1 hdac1 (Catalog #AAA61568) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Histone Deacetylase 1 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Histone Deacetylase 1 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the HDAC1 hdac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KPEAKGVKEE VK. It is sometimes possible for the material contained within the vial of "Histone Deacetylase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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