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IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA229431 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human Histone deacetylase 3 Polyclonal Antibody | anti-HDAC3 antibody
Rabbit anti-human Histone deacetylase 3 polyclonal Antibody
Gene Names
HDAC3; HD3; RPD3; RPD3-2
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Histone deacetylase 3, Antibody; Rabbit anti-human Histone deacetylase 3 polyclonal Antibody; HD3; RPD3-2; SMAP45; HDAC3; anti-HDAC3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
428
Applicable Applications for anti-HDAC3 antibody
ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Histone deacetylase 3 protein(1-428aa)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8138 / sc-11417
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA229431 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA229431 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.2^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-HDAC3 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.2^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-HDAC3 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA229431 at dilution 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA229431 at dilution 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in: mouse heartAll lanes: HDAC3 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49,50 kDaObserved band size: 49 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: mouse heartAll lanes: HDAC3 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49,50 kDaObserved band size: 49 kDa)
WB (Western Blot)
(Western blotAll lanes: Histone deacetylase 3 antibody at 2ug/ml+Hela cellsSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 49,50 kDaObserved band size: 49 kDa)
WB (Western Blot)
(Western blotAll lanes: Histone deacetylase 3 antibody at 2ug/ml+Hela cellsSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 49,50 kDaObserved band size: 49 kDa)
Related Product Information for anti-HDAC3 antibody
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,111 Da
NCBI Official Full Name
histone deacetylase 3
NCBI Official Synonym Full Names
histone deacetylase 3
NCBI Official Symbol
HDAC3
NCBI Official Synonym Symbols
HD3; RPD3; RPD3-2
NCBI Protein Information
histone deacetylase 3
UniProt Protein Name
Histone deacetylase 3
UniProt Gene Name
HDAC3
UniProt Synonym Gene Names
HD3
UniProt Entry Name
HDAC3_HUMAN
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Product Notes
The HDAC3 hdac3 (Catalog #AAA229431) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Histone deacetylase 3 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone deacetylase 3 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HDAC3 hdac3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone deacetylase 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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