DB (Dot Blot)
(Dot Blot testing of histone H2A (pThr120) antibody (AAA50455). Peptides containing phosphorylated threonine 120 of human histone H2A (lane 1) and unmodified human histone H2A (lane 2) were spotted onto PVDF in various amounts (indicated on the left in picomoles) and probed with AAA50455 at a dilution of 1 ug/ml.)
Rabbit anti-Human Histone H2A Polyclonal Antibody | anti-HIST2H2AC antibody
Histone H2A (pThr120) antibody
Gene Names
HIST2H2AC; H2A; H2A/q; H2AFQ; H2A-GL101
Reactivity
Human
Applications
Western Blot, Immunoblot, Western Blot
Purity
Antiserum to human histone H2A (pThr120) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Synonyms
Histone H2A, Antibody; Histone H2A (pThr120) antibody; RABBIT ANTI HUMAN HISTONE H2A (pThr120); anti-HIST2H2AC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antiserum to human histone H2A (pThr120) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Applicable Applications for anti-HIST2H2AC antibody
WB (Western Blot), IB (Immunoblot)
Immunogen
A peptide containing phosphorylated threonine 120 of human histone H2A
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
DB (Dot Blot)
(Dot Blot testing of histone H2A (pThr120) antibody (AAA50455). Peptides containing phosphorylated threonine 120 of human histone H2A (lane 1) and unmodified human histone H2A (lane 2) were spotted onto PVDF in various amounts (indicated on the left in picomoles) and probed with AAA50455 at a dilution of 1 ug/ml.)
DB (Dot Blot)
(Dot Blot testing of histone H2A (pThr120) antibody (AAA50455). Peptides containing phosphorylated threonine 120 of human histone H2A (lane 1) and unmodified human histone H2A (lane 2) were spotted onto PVDF in various amounts (indicated on the left in picomoles) and probed with AAA50455 at a dilution of 1 ug/ml.)
WB (Western Blot)
(Western blot analysis of HeLa acid extract (10 ug; lane 1) and HeLa acid extract treated with colcemid to arrest cells at mitosis (10 ug; lane 2) probed with histone H2A (pThr120) antibody (AAA50455) at a dilution of 1 ug/ml.)
WB (Western Blot)
(Western blot analysis of HeLa acid extract (10 ug; lane 1) and HeLa acid extract treated with colcemid to arrest cells at mitosis (10 ug; lane 2) probed with histone H2A (pThr120) antibody (AAA50455) at a dilution of 1 ug/ml.)
Related Product Information for anti-HIST2H2AC antibody
Rabbit anti Human histone H2A (pThr120) antibody recognizes histone H2A when phosphorylated at threonine 120.
Histone H2A is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Phosphorylation of histone H2A at threonine 120 promotes oncogenic transformation in human cancer cell lines via upregulation of cyclin D1 (Aihara et al. 2016).
Wide species cross-reactivity is expected from Rabbit anti Human histone H2A (pThr120) antibody based on sequence homology.
Histone H2A is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Phosphorylation of histone H2A at threonine 120 promotes oncogenic transformation in human cancer cell lines via upregulation of cyclin D1 (Aihara et al. 2016).
Wide species cross-reactivity is expected from Rabbit anti Human histone H2A (pThr120) antibody based on sequence homology.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,988 Da
NCBI Official Full Name
histone H2A type 2-C
NCBI Official Synonym Full Names
histone cluster 2, H2ac
NCBI Official Symbol
HIST2H2AC
NCBI Official Synonym Symbols
H2A; H2A/q; H2AFQ; H2A-GL101
NCBI Protein Information
histone H2A type 2-C; histone IIa; histone H2A/q; histone 2, H2ac; histone H2A-GL101; H2A histone family, member Q
UniProt Protein Name
Histone H2A type 2-C
UniProt Gene Name
HIST2H2AC
UniProt Synonym Gene Names
H2AFQ
UniProt Entry Name
H2A2C_HUMAN
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Product Notes
The HIST2H2AC hist2h2ac (Catalog #AAA50455) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2A (pThr120) antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IB (Immunoblot). Researchers should empirically determine the suitability of the HIST2H2AC hist2h2ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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