DB (Dot Blot)
(Histone H2AK119ub antibody tested by Dot blot. Lane 1: unmodified Histone H2A. Lane 2: ubiquityl-lysine 119 H2A. Peptides were spotted on membrane and probed with antibody at a dilution of 1:1000.)
Rabbit Histone H2AK119ub Polyclonal Antibody | anti-H2AK119ub antibody
Histone H2AK119ub antibody (pAb)
Gene Names
HIST2H2AC; H2A; H2A/q; H2AFQ; H2A-GL101
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Dot Blot
Purity
Affinity Purified
Synonyms
Histone H2AK119ub, Antibody; Histone H2AK119ub antibody (pAb); anti-H2AK119ub antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol, and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H2AK119ub antibody
WB (Western Blot), DB (Dot Blot)
Immunogen
This antibody was raised against a branched peptide corresponding to the ubiquitin conjugation site at Lys119 of human Histone H2A.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(Histone H2AK119ub antibody tested by Dot blot. Lane 1: unmodified Histone H2A. Lane 2: ubiquityl-lysine 119 H2A. Peptides were spotted on membrane and probed with antibody at a dilution of 1:1000.)
DB (Dot Blot)
(Histone H2AK119ub antibody tested by Dot blot. Lane 1: unmodified Histone H2A. Lane 2: ubiquityl-lysine 119 H2A. Peptides were spotted on membrane and probed with antibody at a dilution of 1:1000.)
SDS-PAGE
(Histone H2AK119ub antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with antibody at a 1:500 dilution.)
SDS-PAGE
(Histone H2AK119ub antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with antibody at a 1:500 dilution.)
Related Product Information for anti-H2AK119ub antibody
Short Description: Our Histone H2AK119ub antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15% of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies.
Background: In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15% of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies.
Product Categories/Family for anti-H2AK119ub antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14kDa
NCBI Official Full Name
histone H2A type 2-C
NCBI Official Synonym Full Names
histone cluster 2 H2A family member c
NCBI Official Symbol
HIST2H2AC
NCBI Official Synonym Symbols
H2A; H2A/q; H2AFQ; H2A-GL101
NCBI Protein Information
histone H2A type 2-C
UniProt Protein Name
Histone H2A type 2-C
UniProt Gene Name
HIST2H2AC
UniProt Synonym Gene Names
H2AFQ
UniProt Entry Name
H2A2C_HUMAN
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Product Notes
The H2AK119ub hist2h2ac (Catalog #AAA60097) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2AK119ub antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2AK119ub can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), DB (Dot Blot). Researchers should empirically determine the suitability of the H2AK119ub hist2h2ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2AK119ub, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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