IF (Immunofluorescence)
(Detection of Histone H2AK5ac by immunofluorescence. U2OS cells were stained with H2AK5ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2AK5ac antibody staining. Right panel: merge.)
Rabbit Histone H2AK5ac Polyclonal Antibody | anti-H2AK5ac antibody
Histone H2AK5ac antibody (pAb)
Gene Names
HIST2H2AC; H2A; H2A/q; H2AFQ; H2A-GL101
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Synonyms
Histone H2AK5ac, Antibody; Histone H2AK5ac antibody (pAb); anti-H2AK5ac antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H2AK5ac antibody
WB (Western Blot), IF (Immunofluorescence), DB (Dot Blot), ChIP (Chromatin immunoprecipitation)
Conjugation
Acetylated
Immunogen
This Histone H2A acetyl Lys5 antibody was raised against a peptide including acetyl-lysine 5 of human histone H2A.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
IF (Immunofluorescence)
(Detection of Histone H2AK5ac by immunofluorescence. U2OS cells were stained with H2AK5ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2AK5ac antibody staining. Right panel: merge.)
IF (Immunofluorescence)
(Detection of Histone H2AK5ac by immunofluorescence. U2OS cells were stained with H2AK5ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2AK5ac antibody staining. Right panel: merge.)
DB (Dot Blot)
(Specificity data:. Dot blot analysis was used to confirm the specificity of Histone H2A acetyl Lys5 pAb for acetyl Lys5 of Histone H2A. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:2,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Peptide acetylated at Lys5 of H2A. Lane 2: Unmodified Lys5 peptide. Lane 3: Peptide acetylated at Lys9 of H2A. Lane 4: Unmodified Lys9 peptide.)
DB (Dot Blot)
(Specificity data:. Dot blot analysis was used to confirm the specificity of Histone H2A acetyl Lys5 pAb for acetyl Lys5 of Histone H2A. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:2,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Peptide acetylated at Lys5 of H2A. Lane 2: Unmodified Lys5 peptide. Lane 3: Peptide acetylated at Lys9 of H2A. Lane 4: Unmodified Lys9 peptide.)
WB (Western Blot)
(Histone H2A acetyl Lys5 pAb tested by Western blot HeLa acid extract (5 ug per lane) was probed with Histone H2A acetyl Lys5 polyclonal antibody at a 1:2,000 dilution. Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)
WB (Western Blot)
(Histone H2A acetyl Lys5 pAb tested by Western blot HeLa acid extract (5 ug per lane) was probed with Histone H2A acetyl Lys5 polyclonal antibody at a 1:2,000 dilution. Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)
Related Product Information for anti-H2AK5ac antibody
Short Description: Our Histone H2AK5ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A Lys5 is preferentially acetylated by TIP60 and deacetylated by HDAC3.
Background: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A Lys5 is preferentially acetylated by TIP60 and deacetylated by HDAC3.
Product Categories/Family for anti-H2AK5ac antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14kDa
NCBI Official Full Name
histone H2A type 2-C
NCBI Official Synonym Full Names
histone cluster 2 H2A family member c
NCBI Official Symbol
HIST2H2AC
NCBI Official Synonym Symbols
H2A; H2A/q; H2AFQ; H2A-GL101
NCBI Protein Information
histone H2A type 2-C
UniProt Protein Name
Histone H2A type 2-C
UniProt Gene Name
HIST2H2AC
UniProt Synonym Gene Names
H2AFQ
UniProt Entry Name
H2A2C_HUMAN
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Product Notes
The H2AK5ac hist2h2ac (Catalog #AAA59827) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2AK5ac antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2AK5ac can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), DB (Dot Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H2AK5ac hist2h2ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2AK5ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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