DB (Dot Blot)
(Histone H2A phospho Thr120 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A phospho Thr120 pAb for phospho Thr120 histone H2A. The modified and unmodified peptides to the immunogen were spotted onto PVDF and probed with the antibody at a dilution of 1 ug/ml . The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Phospho Thr120 peptide. Lane 2: Unmodified Thr120 peptide.)
Rabbit Histone H2AT120ph Polyclonal Antibody | anti-H2AT120ph antibody
Histone H2AT120ph antibody (pAb)
Gene Names
HIST2H2AC; H2A; H2A/q; H2AFQ; H2A-GL101
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Dot Blot
Purity
Protein A Chromatography
Synonyms
Histone H2AT120ph, Antibody; Histone H2AT120ph antibody (pAb); anti-H2AT120ph antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version of this antibody is also available.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H2AT120ph antibody
WB (Western Blot), IF (Immunofluorescence), DB (Dot Blot)
Conjugation
Phosphorylated
Immunogen
This Histone H2A phospho Thr120 antibody was raised against a peptide containing phospho Thr120 of human histone H2A.
Abs Residue
Thr
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(Histone H2A phospho Thr120 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A phospho Thr120 pAb for phospho Thr120 histone H2A. The modified and unmodified peptides to the immunogen were spotted onto PVDF and probed with the antibody at a dilution of 1 ug/ml . The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Phospho Thr120 peptide. Lane 2: Unmodified Thr120 peptide.)
DB (Dot Blot)
(Histone H2A phospho Thr120 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A phospho Thr120 pAb for phospho Thr120 histone H2A. The modified and unmodified peptides to the immunogen were spotted onto PVDF and probed with the antibody at a dilution of 1 ug/ml . The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Phospho Thr120 peptide. Lane 2: Unmodified Thr120 peptide.)
WB (Western Blot)
(Histone H2A phospho Thr120 pAb tested by Western blot. HeLa acid extract (10 ug per lane) was probed with Histone H2A phospho Thr120 pAb at a dilution of 1 ug/ml . Lane 1: No treatment. Lane 2: Cells treated with colcemid to arrest cells at mitosis.)
WB (Western Blot)
(Histone H2A phospho Thr120 pAb tested by Western blot. HeLa acid extract (10 ug per lane) was probed with Histone H2A phospho Thr120 pAb at a dilution of 1 ug/ml . Lane 1: No treatment. Lane 2: Cells treated with colcemid to arrest cells at mitosis.)
Related Product Information for anti-H2AT120ph antibody
Short Description: Our Histone H2AT120ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Phosphorylation of histones occurs at multiple sites during mitosis. H2A Thr120 phosphorylation is observed on chromatin during both mitosis and meiosis. Thr120 phosphorylation is inversely correlated with ubiquitylation of H2A Lys119 in meiotic mouse spermatocytes. In Drosophila, loss of H2A Thr120 phosphorylation is associated with a failure to disassemble the synaptonemal complex, impaired loading of condensin and female infertility. It is possible that H2A Thr120 phosphorylation is involved in the regulation of chromatin structure.
Background: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Phosphorylation of histones occurs at multiple sites during mitosis. H2A Thr120 phosphorylation is observed on chromatin during both mitosis and meiosis. Thr120 phosphorylation is inversely correlated with ubiquitylation of H2A Lys119 in meiotic mouse spermatocytes. In Drosophila, loss of H2A Thr120 phosphorylation is associated with a failure to disassemble the synaptonemal complex, impaired loading of condensin and female infertility. It is possible that H2A Thr120 phosphorylation is involved in the regulation of chromatin structure.
Product Categories/Family for anti-H2AT120ph antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14kDa
NCBI Official Full Name
histone H2A type 2-C
NCBI Official Synonym Full Names
histone cluster 2 H2A family member c
NCBI Official Symbol
HIST2H2AC
NCBI Official Synonym Symbols
H2A; H2A/q; H2AFQ; H2A-GL101
NCBI Protein Information
histone H2A type 2-C
UniProt Protein Name
Histone H2A type 2-C
UniProt Gene Name
HIST2H2AC
UniProt Synonym Gene Names
H2AFQ
UniProt Entry Name
H2A2C_HUMAN
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Product Notes
The H2AT120ph hist2h2ac (Catalog #AAA59993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2AT120ph antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2AT120ph can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), DB (Dot Blot). Researchers should empirically determine the suitability of the H2AT120ph hist2h2ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2AT120ph, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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