WB (Western Blot)
(Histone H2AX phospho Ser139 antibody tested by Western blot. Western blot: Nuclear extract of U2OS cells (20 ug per lane) probed with Histone H2AX phospho Ser139 polyclonal antibody (1:500 dilution). Lane 1: untreated cells Lane 2: cells treated with camptothecin)
Rabbit Histone H2A.XS139ph (H2AX) Polyclonal Antibody | anti-H2A.XS139ph antibody
Histone H2A.XS139ph antibody (pAb)(H2AX)
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Dot Blot
Purity
Protein A Chromatography
Synonyms
Histone H2A.XS139ph (H2AX), Antibody; Histone H2A.XS139ph antibody (pAb)(H2AX); Histone H2A.X phosphoserine 139; anti-H2A.XS139ph antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
PBS containing 30% glycerol, 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H2A.XS139ph antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot)
Conjugation
Phosphorylated
Immunogen
This Histone H2AX phospho Ser139 antibody was raised against a peptide including phosphoserine 139 of histone H2AX.
Abs Residue
Ser
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Histone H2AX phospho Ser139 antibody tested by Western blot. Western blot: Nuclear extract of U2OS cells (20 ug per lane) probed with Histone H2AX phospho Ser139 polyclonal antibody (1:500 dilution). Lane 1: untreated cells Lane 2: cells treated with camptothecin)
WB (Western Blot)
(Histone H2AX phospho Ser139 antibody tested by Western blot. Western blot: Nuclear extract of U2OS cells (20 ug per lane) probed with Histone H2AX phospho Ser139 polyclonal antibody (1:500 dilution). Lane 1: untreated cells Lane 2: cells treated with camptothecin)
IF (Immunofluorescence)
(Histone H2AX phospho Ser139 antibody tested by immunofluorescence. Etoposide-treated HeLa cells stained with (1:500 dilution, red) and counterstained with DAPI (blue).)
IF (Immunofluorescence)
(Histone H2AX phospho Ser139 antibody tested by immunofluorescence. Etoposide-treated HeLa cells stained with (1:500 dilution, red) and counterstained with DAPI (blue).)
IF (Immunofluorescence)
(Histone H2AX phospho Ser139 antibody tested by immunofluorescence. HeLa cells stained with Histone H2AX phospho Ser139 antibody (1:500 dilution) using MAX Stain Immunofluorescence Tools. The HeLa cells were blocked with MAXblock Blocking Medium and stained with Histone H2AX phospho Ser139 antibody. Panel A: Untreated HeLa cells. Panel B: Cells fixed and stained 90 minutes after 3 Gy ionizing radiation treatment. Top images: Cells were stained with DAPI. Middle images: Same cells stained with Histone H2AX phospho Ser139 antibody. Bottom images: Merge of both images above. Images were made using Zeiss Axiovision with equivalent acquisition settings for direct comparison. Note Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of Ser139 of H2AX. In contrast, Panel A-middle image shows no detectable phosphorylated H2AX.)
IF (Immunofluorescence)
(Histone H2AX phospho Ser139 antibody tested by immunofluorescence. HeLa cells stained with Histone H2AX phospho Ser139 antibody (1:500 dilution) using MAX Stain Immunofluorescence Tools. The HeLa cells were blocked with MAXblock Blocking Medium and stained with Histone H2AX phospho Ser139 antibody. Panel A: Untreated HeLa cells. Panel B: Cells fixed and stained 90 minutes after 3 Gy ionizing radiation treatment. Top images: Cells were stained with DAPI. Middle images: Same cells stained with Histone H2AX phospho Ser139 antibody. Bottom images: Merge of both images above. Images were made using Zeiss Axiovision with equivalent acquisition settings for direct comparison. Note Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of Ser139 of H2AX. In contrast, Panel A-middle image shows no detectable phosphorylated H2AX.)
Related Product Information for anti-H2A.XS139ph antibody
Short Description: Our Histone H2A.XS139ph antibody (pAb)(H2AX) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.
Background: Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.
Product Categories/Family for anti-H2A.XS139ph antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,145 Da
NCBI Official Full Name
histone H2AX
NCBI Official Synonym Full Names
H2A histone family, member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
NCBI Protein Information
histone H2AX; H2AX histone; histone H2A.x
UniProt Protein Name
Histone H2A.x
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
UniProt Entry Name
H2AX_HUMAN
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Product Notes
The H2A.XS139ph h2afx (Catalog #AAA59831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2A.XS139ph antibody (pAb)(H2AX) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A.XS139ph (H2AX) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot). Researchers should empirically determine the suitability of the H2A.XS139ph h2afx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A.XS139ph (H2AX), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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