Rabbit Histone H3K4me3 Polyclonal Antibody | anti-H3K4me3 antibody

Histone H3K4me3 antibody (pAb)

Cat. Num. AAA59808

• Reactivity: Budding Yeast, Human, Mouse, Wide Range Predicted
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Synonyms: Histone H3K4me3, Antibody; Histone H3K4me3 antibody (pAb); anti-H3K4me3 antibody

• Host: Rabbit
• Reactivity: Budding Yeast, Human, Mouse, Wide Range Predicted
• Clonality: Polyclonal
• Form/Format: Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version of this antibody that was purified by Protein A Chromatography is also available.

• Applicable Applications for anti-H3K4me3 antibody: WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
• Conjugation: Methylated
• Immunogen: This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3.
• Abs Residue: Lys
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

DB (Dot Blot)

(Histone H3K4me3 antibody (pAb) tested by dot blot analysis. Dot blot was used to confirm specificity of Histone H3K4me3 antibody. Peptides corresponding to regions around major sites of histone H3 methylation (lysine 4, lysine 9, lysine 27) were spotted onto PVDF and probed with antibody at a dilution of 1:500. The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified H3K4. Lane 2: H3K4me1. Lane 3: H3K4me2. Lane 4: H3K4me3. Lane 5: unmodified H3K9. Lane 6: H3K9me1. Lane 7: H3K9me2. Lane 8: H3K9me3. Lane 9: unmodified H3K79. Lane 10: H3K79me1. Lane 11: H3K79me2. Lane 12: H3K79me3. Bottom panel - Lane 1: unmodified H3K23. Lane 2: H3K23me1. Lane 3: H3K23me3. Lane 4: H3K23me3. Lane 5: unmodified H3K27. Lane 6: H3K27me1. Lane 7: H3K27me2. Lane 8: H3K27me3. Lane 9: unmodified H3K36. Lane 10: H3K36me1. Lane 11: H3K36me2. Lane 12: H3K36me3.)

WB (Western Blot)

(Histone H3K4me3 antibody (pAb) tested by Western blot. HeLa nuclear extract (20 ug) was probed with Histone H3K4me3 antibody (pAb) at a 1:1000 dilution. Note: For optimal results, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. It is also recommended to include 0.05% Tween 20 in all blocking solutions to reduce background. Individual optimization may be required.)

IF (Immunofluorescence)

(Histone H3 trimethyl Lys4 antibody tested by immunofluorescence. HeLa cells stained at 1:1,000 with Histone H3 trimethyl Lys4 antibody. Top left: Histone H3 trimethyl Lys4 antibody.Top right: Stained with DAPI. Bottom left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom right: Merge of all 3 images.)

ChIP (Chromatin Immunoprecipitation)

(Histone H3 trimethyl Lys4 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 2 million HL-60 cells and 3 ul of Histone H3 trimethyl Lys4 antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)

ChIP (Chromatin Immunoprecipitation)

(Histone H3K4me3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug chromatin from human acute myelocytic leukemia cells and 3 ul of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 12.1 million sequence tags were mapped to identify H3K4me3 binding sites on chromosome 12.)

Related Product Information for anti-H3K4me3 antibody

Short Description: Our Histone H3K4me3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, CUT&Tag, Dot blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western blot; it has been shown to react with Budding Yeast, Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Product Categories/Family for anti-H3K4me3 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• Molecular Weight: 17kDa