DB (Dot Blot)
(Histone H3 phospho Thr11 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr11 pAb for phospho-Thr11 of histone H3. Decreasing amounts of peptides corresponding to regions around major sites of histone H3 threonine phosphorylation were spotted onto PVDF and probed with the antibody at a dilution of 0.5 ug/ml. Lane 1: Peptide phosphorylated at threonine 11. Lane 2: Unmodified threonine 11 peptide. Lane 3: Peptide phosphorylated at threonine 3. Lane 4: Peptide phosphorylated at threonine 6. Lane 5: Peptide phosphorylated at threonine 45. Lane 6: Peptide phosphorylated at threonine 80. Lane 7:Peptide phosphorylated at threonine 10. Lane 8:Peptide phosphorylated at threonine 28.)
Rabbit Histone H3T11ph Polyclonal Antibody | anti-H3T11ph antibody
Histone H3T11ph antibody (pAb)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Dot Blot
Purity
Protein A Chromatography
Synonyms
Histone H3T11ph, Antibody; Histone H3T11ph antibody (pAb); anti-H3T11ph antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version of this antibody is also available.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3T11ph antibody
WB (Western Blot), DB (Dot Blot)
Conjugation
Phosphorylated
Immunogen
This Histone H3 phospho Thr11 antibody was raised against a peptide containing phospho-Thr11 of human histone H3.
Abs Residue
Thr
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(Histone H3 phospho Thr11 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr11 pAb for phospho-Thr11 of histone H3. Decreasing amounts of peptides corresponding to regions around major sites of histone H3 threonine phosphorylation were spotted onto PVDF and probed with the antibody at a dilution of 0.5 ug/ml. Lane 1: Peptide phosphorylated at threonine 11. Lane 2: Unmodified threonine 11 peptide. Lane 3: Peptide phosphorylated at threonine 3. Lane 4: Peptide phosphorylated at threonine 6. Lane 5: Peptide phosphorylated at threonine 45. Lane 6: Peptide phosphorylated at threonine 80. Lane 7:Peptide phosphorylated at threonine 10. Lane 8:Peptide phosphorylated at threonine 28.)
DB (Dot Blot)
(Histone H3 phospho Thr11 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr11 pAb for phospho-Thr11 of histone H3. Decreasing amounts of peptides corresponding to regions around major sites of histone H3 threonine phosphorylation were spotted onto PVDF and probed with the antibody at a dilution of 0.5 ug/ml. Lane 1: Peptide phosphorylated at threonine 11. Lane 2: Unmodified threonine 11 peptide. Lane 3: Peptide phosphorylated at threonine 3. Lane 4: Peptide phosphorylated at threonine 6. Lane 5: Peptide phosphorylated at threonine 45. Lane 6: Peptide phosphorylated at threonine 80. Lane 7:Peptide phosphorylated at threonine 10. Lane 8:Peptide phosphorylated at threonine 28.)
WB (Western Blot)
(Histone H3 phospho Thr11 pAb tested by Western blot. Western blot probed with Histone H3 phospho Thr11 pAb (1 ug/ml dilution). Lane 1: 20 ug nuclear extract of HeLa cells. Lane 2: 20 ug nuclear extract of HeLa cells treated with colcemid.)
WB (Western Blot)
(Histone H3 phospho Thr11 pAb tested by Western blot. Western blot probed with Histone H3 phospho Thr11 pAb (1 ug/ml dilution). Lane 1: 20 ug nuclear extract of HeLa cells. Lane 2: 20 ug nuclear extract of HeLa cells treated with colcemid.)
Related Product Information for anti-H3T11ph antibody
Short Description: Our Histone H3T11ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Phosphorylation of several residues of histone H3, such as Ser10 (Histone H3 phospho Ser10), Ser28 (Histone H3 phospho Ser28) and Thr11, is tightly correlated with chromosome condensation during both mitosis and meiosis. Localization of histone H3 phospho Thr11 is different in mammals and in plants. In plant cells, phosphorylation of Thr11 of histone H3 is distributed along the entire length of condensed chromosome. In mammals, this modification is restricted to the centromeric region of the chromosome. Thr11 of histone H3 is phosphorylated by Dlk/ZIP kinases during mitosis.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Phosphorylation of several residues of histone H3, such as Ser10 (Histone H3 phospho Ser10), Ser28 (Histone H3 phospho Ser28) and Thr11, is tightly correlated with chromosome condensation during both mitosis and meiosis. Localization of histone H3 phospho Thr11 is different in mammals and in plants. In plant cells, phosphorylation of Thr11 of histone H3 is distributed along the entire length of condensed chromosome. In mammals, this modification is restricted to the centromeric region of the chromosome. Thr11 of histone H3 is phosphorylated by Dlk/ZIP kinases during mitosis.
Product Categories/Family for anti-H3T11ph antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,367 Da
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 4, H4
NCBI Official Symbol
HIST4H4
NCBI Official Synonym Symbols
H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
NCBI Protein Information
histone H4; histone 4, H4; OTTHUMP00000239323
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
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Product Notes
The H3T11ph hist1h4a (Catalog #AAA59994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3T11ph antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3T11ph can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), DB (Dot Blot). Researchers should empirically determine the suitability of the H3T11ph hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3T11ph, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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