WB (Western Blot)
(Western blot analysis of untreated HeLa nuclear extract (20 ug; lane 1) or HeLa nuclear extract treated with sodium butyrate (lane 2) probed with histone H4 (Ac12) antibody (AAA50459) at a 1 ug/ml dilution.)
Rabbit anti-Human Histone H4 Polyclonal Antibody | anti-HIST2H4B antibody
Histone H4 (Ac12) antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Antiserum to human histone H4 (Ac12) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Synonyms
Histone H4, Antibody; Histone H4 (Ac12) antibody; RABBIT ANTI HUMAN HISTONE H4 (Ac12); anti-HIST2H4B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antiserum to human histone H4 (Ac12) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Applicable Applications for anti-HIST2H4B antibody
WB (Western Blot), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation)
Species Cross-Reactivity
Mouse:Yes
Immunogen
A peptide including acetylated lysine 12 of histone H4
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
WB (Western Blot)
(Western blot analysis of untreated HeLa nuclear extract (20 ug; lane 1) or HeLa nuclear extract treated with sodium butyrate (lane 2) probed with histone H4 (Ac12) antibody (AAA50459) at a 1 ug/ml dilution.)
WB (Western Blot)
(Western blot analysis of untreated HeLa nuclear extract (20 ug; lane 1) or HeLa nuclear extract treated with sodium butyrate (lane 2) probed with histone H4 (Ac12) antibody (AAA50459) at a 1 ug/ml dilution.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells probed with histone H4 (Ac12) antibody (AAA50459) at a 2 ug/ml dilution (top) and DAPI (middle). Bottom panel shows merged images..)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells probed with histone H4 (Ac12) antibody (AAA50459) at a 2 ug/ml dilution (top) and DAPI (middle). Bottom panel shows merged images..)
Related Product Information for anti-HIST2H4B antibody
Rabbit anti Human histone H4 (Ac12) antibody recognizes histone H4 when acetylated at lysine 12.
Histone H4 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation (Koch et al. 2007). Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions (Kuo and Allis, 1998). Acetylation of histone H4 usually results in transcriptional activation (Turner et al. 1989).
Wide species cross-reactivity is expected from Rabbit anti Human histone H4 (Ac12) antibody based on sequence.
Histone H4 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation (Koch et al. 2007). Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions (Kuo and Allis, 1998). Acetylation of histone H4 usually results in transcriptional activation (Turner et al. 1989).
Wide species cross-reactivity is expected from Rabbit anti Human histone H4 (Ac12) antibody based on sequence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,367 Da
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 2, H4b
NCBI Official Symbol
HIST2H4B
NCBI Official Synonym Symbols
H4/o
NCBI Protein Information
histone H4; histone 2, H4b
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
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Product Notes
The HIST2H4B hist1h4a (Catalog #AAA50459) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H4 (Ac12) antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the HIST2H4B hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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