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DB (Dot Blot)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 pan-acetyl antibody for acetyl histone H4. Column 1: unacetylated histone H4 peptide. Column 2: acetylated peptide corresponding to immunogen. Peptides were spotted onto PVDF and probed with Histone H4 pan-acetyl antibody at 1 ug /ml. The amount of peptide (picomoles) spotted is indicated next to each row.)
Rabbit Histone H4ac Polyclonal Antibody | anti-H4ac antibody
Histone H4ac (pan-acetyl) antibody (pAb)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
Histone H4ac, Antibody; Histone H4ac (pan-acetyl) antibody (pAb); anti-H4ac antibody
Host
Rabbit
Reactivity
Human, Mouse, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS (pH7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version of this antibody is also available.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H4ac antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Conjugation
Acetylated
Immunogen
This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena hv1, an H2A variant with homology to mammalian histone H4. The antibody recognizes acetylated histone H4, but not H2A, in HeLa extracts.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 pan-acetyl antibody for acetyl histone H4. Column 1: unacetylated histone H4 peptide. Column 2: acetylated peptide corresponding to immunogen. Peptides were spotted onto PVDF and probed with Histone H4 pan-acetyl antibody at 1 ug /ml. The amount of peptide (picomoles) spotted is indicated next to each row.)
DB (Dot Blot)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 pan-acetyl antibody for acetyl histone H4. Column 1: unacetylated histone H4 peptide. Column 2: acetylated peptide corresponding to immunogen. Peptides were spotted onto PVDF and probed with Histone H4 pan-acetyl antibody at 1 ug /ml. The amount of peptide (picomoles) spotted is indicated next to each row.)
WB (Western Blot)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H4 pan-acetyl (1 ug per ml). Lane 1: no treatment. Lane 2: cells treated with sodium butyrate.)
WB (Western Blot)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H4 pan-acetyl (1 ug per ml). Lane 1: no treatment. Lane 2: cells treated with sodium butyrate.)
IF (Immunofluorescence)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by immunofluorescence. HeLa cells stained at 2 ug/ml with Histone H4 pan-acetyl antibody. Top panel: Histone H4 pan-acetyl antibody. Middle panel: DAPI. Bottom panel: merge.)
IF (Immunofluorescence)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by immunofluorescence. HeLa cells stained at 2 ug/ml with Histone H4 pan-acetyl antibody. Top panel: Histone H4 pan-acetyl antibody. Middle panel: DAPI. Bottom panel: merge.)
ChIP (Chromatin Immunoprecipitation)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 ug of Histone H4 pan-acetyl antibody or the equivalent amount of rabbit IgG as a negative control. Real-time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
ChIP (Chromatin Immunoprecipitation)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 ug of Histone H4 pan-acetyl antibody or the equivalent amount of rabbit IgG as a negative control. Real-time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
ChIP (Chromatin Immunoprecipitation)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 15 ug of chromatin from a human medulloblastoma cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 12 million sequence tags were mapped to identify Histone H4ac (pan-acetyl) binding sites. The image shows binding across a region of chromosome 12.)
ChIP (Chromatin Immunoprecipitation)
(Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 15 ug of chromatin from a human medulloblastoma cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 12 million sequence tags were mapped to identify Histone H4ac (pan-acetyl) binding sites. The image shows binding across a region of chromosome 12.)
Related Product Information for anti-H4ac antibody
Short Description: Our Histone H4ac (pan-acetyl) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western blot; it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.
Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.
Product Categories/Family for anti-H4ac antibody
NCBI and Uniprot Product Information
Molecular Weight
8kDa
Similar Products
Product Notes
The H4ac (Catalog #AAA59796) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H4ac (pan-acetyl) antibody (pAb) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4ac can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H4ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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