DB (Dot Blot)
(Histone H4 dimethyl Arg3 symmetric pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 symmetric pAb for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 symmetric pAb at 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified Arg3. Lane 2: dimethyl-Arg3 H4 (symmetric). Lane 3: dimethyl-Arg3 H4 (asymmetric). Lane 4: monomethyl-Arg3 H4. Lane 5: unmodified Arg26 H3. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (asymmetric). Lane 8: dimethyl-Arg26 H3 (symmetric). Lane 9: dimethyl-Arg2 H3 (asymmetric). Lane 10: dimethyl-Arg2 (symmetric). Lane 11: dimethyl-Arg8 H3 (asymmetric). Lane 12: dimethyl-Arg8 H3 (symmetric). Lane 13: dimethyl-Arg17 (asymmetric). Lane 14: dimethyl-Arg17 H3 (symmetric).)
Rabbit Histone H4R3me2s Polyclonal Antibody | anti-H4R3me2s antibody
Histone H4R3me2s (symmetric) antibody (pAb)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
Histone H4R3me2s, Antibody; Histone H4R3me2s (symmetric) antibody (pAb); anti-H4R3me2s antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H4R3me2s antibody
WB (Western Blot), DB (Dot Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Conjugation
Methylated
Immunogen
This Histone H4 dimethyl Arg3 (H3R3me2s) antibody was raised against a peptide containing dimethyl Arg3 (symmetric) of human Histone H4.
Abs Residue
Arg
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(Histone H4 dimethyl Arg3 symmetric pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 symmetric pAb for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 symmetric pAb at 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified Arg3. Lane 2: dimethyl-Arg3 H4 (symmetric). Lane 3: dimethyl-Arg3 H4 (asymmetric). Lane 4: monomethyl-Arg3 H4. Lane 5: unmodified Arg26 H3. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (asymmetric). Lane 8: dimethyl-Arg26 H3 (symmetric). Lane 9: dimethyl-Arg2 H3 (asymmetric). Lane 10: dimethyl-Arg2 (symmetric). Lane 11: dimethyl-Arg8 H3 (asymmetric). Lane 12: dimethyl-Arg8 H3 (symmetric). Lane 13: dimethyl-Arg17 (asymmetric). Lane 14: dimethyl-Arg17 H3 (symmetric).)
DB (Dot Blot)
(Histone H4 dimethyl Arg3 symmetric pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 symmetric pAb for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 symmetric pAb at 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified Arg3. Lane 2: dimethyl-Arg3 H4 (symmetric). Lane 3: dimethyl-Arg3 H4 (asymmetric). Lane 4: monomethyl-Arg3 H4. Lane 5: unmodified Arg26 H3. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (asymmetric). Lane 8: dimethyl-Arg26 H3 (symmetric). Lane 9: dimethyl-Arg2 H3 (asymmetric). Lane 10: dimethyl-Arg2 (symmetric). Lane 11: dimethyl-Arg8 H3 (asymmetric). Lane 12: dimethyl-Arg8 H3 (symmetric). Lane 13: dimethyl-Arg17 (asymmetric). Lane 14: dimethyl-Arg17 H3 (symmetric).)
WB (Western Blot)
(Histone H4 dimethyl Arg3 symmetric pAb tested by Western blot. Western blot performed using Histone H4 dimethyl Arg3 symmetric pAb at a dilution of 1:2,000. Lane 1: Nuclear extract from Raji cells (20 ug). Lane 2: Nuclear extract from HeLa cells (20 ug).)
WB (Western Blot)
(Histone H4 dimethyl Arg3 symmetric pAb tested by Western blot. Western blot performed using Histone H4 dimethyl Arg3 symmetric pAb at a dilution of 1:2,000. Lane 1: Nuclear extract from Raji cells (20 ug). Lane 2: Nuclear extract from HeLa cells (20 ug).)
Related Product Information for anti-H4R3me2s antibody
Short Description: Our Histone H4R3me2s (symmetric) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Symmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional repression. Histone H4 dimethyl Arg3 symmetric methylation (H4R2me2s) is catalyzed by PRMT5 and PRMT7.
Background: Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Symmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional repression. Histone H4 dimethyl Arg3 symmetric methylation (H4R2me2s) is catalyzed by PRMT5 and PRMT7.
Product Categories/Family for anti-H4R3me2s antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,367 Da
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 4, H4
NCBI Official Symbol
HIST4H4
NCBI Official Synonym Symbols
H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
NCBI Protein Information
histone H4; histone 4, H4; OTTHUMP00000239323
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
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Product Notes
The H4R3me2s hist1h4a (Catalog #AAA59990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H4R3me2s (symmetric) antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4R3me2s can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), DB (Dot Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H4R3me2s hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4R3me2s, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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