Rabbit anti-Human HMGB-1 Polyclonal Antibody | anti-HMGB-1 antibody

HMGB-1 Polyclonal Antibody

Cat. Num. AAA179803

• Gene Names: HMGB1; HMG1; HMG3; SBP-1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antigen Affinity Purification
• Synonyms: HMGB-1, Antibody; HMGB-1 Polyclonal Antibody; Amphoterin; high mobility group box 1; High mobility group protein 1; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG1; HMG-1; HMG1DKFZp686A04236; HMG3; HMGB1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; anti-HMGB-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-HMGB-1 antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant Human HMGB-1 protein expressed by E coli
• Preparation and Storage: Store at -20 degree C Valid for 12 months. Avoid freeze/thaw cycles.

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded Human colon cancer using HMGB-1 Polyclonal Antibody at dilution of 1:350)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human lung cancer using HMGB-1 Polyclonal Antibody at dilution of 1:350)

Related Product Information for anti-HMGB-1 antibody

Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. Residues 27?43 and 178?184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. The cytokine activity of HBMG1 is restricted to the HMG B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4. Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1, respectively.

Product Categories/Family for anti-HMGB-1 antibody

Polyclonal Antibodies; Primary Antibodies; Polyclonal Antibodies; Cancer; Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4504425
• NCBI GeneID: 3146
• NCBI Accession #: NP_002119.1
• NCBI GenBank Nucleotide #: NM_002128.4
• UniProt Accession #: P09429; Q14321; Q5T7C3; Q6IBE1; A5D8W9
• Molecular Weight: 24,894 Da
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: HMGB1
• NCBI Official Synonym Symbols: HMG1; HMG3; SBP-1
• NCBI Protein Information: high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
• UniProt Protein Name: High mobility group protein B1
• UniProt Gene Name: HMGB1
• UniProt Synonym Gene Names: HMG1; HMG-1
• UniProt Entry Name: HMGB1_HUMAN