IP (Immunoprecipitation)
(Detection of human HMGN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGN3 antibody AAA211350 (lot AAA211350-2) used for IP at 6 ug per reaction. HMGN3 was also immunoprecipitated with a previous lot of this antibody AAA211350-1 and rabbit anti-HMGN3 antibody (lots and For blotting immunoprecipitated HGMN3, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human HMGN3 Polyclonal Antibody | anti-HMGN3 antibody
Rabbit anti-HMGN3 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
HMGN3, Antibody; Rabbit anti-HMGN3 Antibody, Affinity Purified; PNAS-25; TRIP7; TR-interacting protein 7; TRIP-7; PNAS-24; high mobility group nucleosome-binding domain-containing protein 3; Thyroid receptor-interacting protein 7; thyroid hormone receptor interacting protein 7; High mobility group nucleosome-binding domain-containing protein 3; high mobility group nucleosomal binding domain 3; anti-HMGN3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-HMGN3 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Orangutan
Immunogen
Between 49 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human HMGN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGN3 antibody AAA211350 (lot AAA211350-2) used for IP at 6 ug per reaction. HMGN3 was also immunoprecipitated with a previous lot of this antibody AAA211350-1 and rabbit anti-HMGN3 antibody (lots and For blotting immunoprecipitated HGMN3, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human HMGN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGN3 antibody AAA211350 (lot AAA211350-2) used for IP at 6 ug per reaction. HMGN3 was also immunoprecipitated with a previous lot of this antibody AAA211350-1 and rabbit anti-HMGN3 antibody (lots and For blotting immunoprecipitated HGMN3, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human HMGN3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HMGN3 (Cat. No. AAA211350 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human HMGN3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HMGN3 (Cat. No. AAA211350 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-HMGN3 antibody
HMGN3 was originally identified in a screen for interaction partners of the thyroid hormone receptor. HMGN3 belongs to the HMGN family of small basic proteins that interacts specifically with nucleosomes and functions to facilitate chromatin unfolding to allow access of transcription factors to DNA targets. HMGN has been found to be a regulator of the glycine transporter 1 (GLYT1) gene.
Product Categories/Family for anti-HMGN3 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HMGN3 hmgn3 (Catalog #AAA211350) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HMGN3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HMGN3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the HMGN3 hmgn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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