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WB (Western Blot)
(Detection of human hnRNP-L by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody AAA212355 (lot AAA212355-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse hnRNP-L Polyclonal Antibody | anti-hnRNP-L antibody
Rabbit anti-hnRNP-L Antibody, Affinity Purified
Gene Names
HNRNPL; HNRPL; hnRNP-L; P/OKcl.14
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
hnRNP-L, Antibody; Rabbit anti-hnRNP-L Antibody, Affinity Purified; hnRNP-L; HNRPL; hnRNP L; heterogeneous nuclear ribonucleoprotein L; epididymis secretory sperm binding protein; P/OKcl.14; Heterogeneous nuclear ribonucleoprotein L; anti-hnRNP-L antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-hnRNP-L antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 350 and 400
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human hnRNP-L by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody AAA212355 (lot AAA212355-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human hnRNP-L by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody AAA212355 (lot AAA212355-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse hnRNP-L by western blot. Samples: Whole cell lysate from mouse NIH 3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody AAA212355 (lot AAA212355-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse hnRNP-L by western blot. Samples: Whole cell lysate from mouse NIH 3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody AAA212355 (lot AAA212355-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human hnRNP-L by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody AAA212355 (lot AAA212355-1) used for IP at 6 ug/mg lysate. hnRNP-L was also immunoprecipitated by rabbit anti-hnRNP-L antibody For blotting immunoprecipitated hnRNP-L, AAA212355 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human hnRNP-L by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody AAA212355 (lot AAA212355-1) used for IP at 6 ug/mg lysate. hnRNP-L was also immunoprecipitated by rabbit anti-hnRNP-L antibody For blotting immunoprecipitated hnRNP-L, AAA212355 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohiostchemistry)
(Detection of mouse hnRNP-L by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-hnRNP-L (Cat. No. AAA212355 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse hnRNP-L by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-hnRNP-L (Cat. No. AAA212355 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human hnRNP-L by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-hnRNP-L (Cat. No. AAA212355 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human hnRNP-L by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-hnRNP-L (Cat. No. AAA212355 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-hnRNP-L antibody
hnRNP-L is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HnRNP-L Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. It associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter cytoplasm [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14866].
Product Categories/Family for anti-hnRNP-L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,561 Da
NCBI Official Full Name
heterogeneous nuclear ribonucleoprotein L isoform a
NCBI Official Synonym Full Names
heterogeneous nuclear ribonucleoprotein L
NCBI Official Symbol
HNRNPL
NCBI Official Synonym Symbols
HNRPL; hnRNP-L; P/OKcl.14
NCBI Protein Information
heterogeneous nuclear ribonucleoprotein L; hnRNP L
UniProt Protein Name
Heterogeneous nuclear ribonucleoprotein L
UniProt Gene Name
HNRNPL
UniProt Synonym Gene Names
HNRPL; hnRNP L
UniProt Entry Name
HNRPL_HUMAN
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Product Notes
The hnRNP-L hnrnpl (Catalog #AAA212355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-hnRNP-L Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's hnRNP-L can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the hnRNP-L hnrnpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hnRNP-L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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