WB (Western Blot)
(Western blot analysis of Rat Brain cell lysates showing detection of HO-1 protein using Rabbit Anti-HO-1 Polyclonal Antibody . Lane 1: Rat Brain lysate. Lane 2: Purified HO-1. Lane 3: Molecular Weight Markers. Load: 10 ug. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:1000.)
Rabbit HO-1 Polyclonal Antibody | anti-Hmox1 antibody
HO-1 Antibody
Gene Names
Hmox1; Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32
Reactivity
Rat, Mouse, Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Synonyms
HO-1, Antibody; HO-1 Antibody; Heme oxygenase 1 antibody; Hsp32 antibody; Hemox antibody; 32 kD antibody; bK286B10 antibody; D8Wsu38e antibody; heat shock protein 32kD antibody; Heat shock protein antibody; Heme oxygenase (decycling) 1 antibody; HMOX 1 antibody; Hmox antibody; HMOX1 an; anti-Hmox1 antibody
Host
Rabbit
Reactivity
Rat, Mouse, Human
Clonality
Polyclonal
Form/Format
Protein A Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
289
Applicable Applications for anti-Hmox1 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Certificate of Analysis
5 ug/mL of SPC-211 was sufficient for detection of HO-1 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Conjugate
Unconjugated
Storage Buffer
PBS pH 7.4, 0.09% azide, 50% glycerol.
Preparation and Storage
-20 degree C
WB (Western Blot)
(Western blot analysis of Rat Brain cell lysates showing detection of HO-1 protein using Rabbit Anti-HO-1 Polyclonal Antibody . Lane 1: Rat Brain lysate. Lane 2: Purified HO-1. Lane 3: Molecular Weight Markers. Load: 10 ug. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:1000.)
WB (Western Blot)
(Western blot analysis of Rat Brain cell lysates showing detection of HO-1 protein using Rabbit Anti-HO-1 Polyclonal Antibody . Lane 1: Rat Brain lysate. Lane 2: Purified HO-1. Lane 3: Molecular Weight Markers. Load: 10 ug. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:1000.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Rabbit Anti-HO-1 Polyclonal Antibody . Tissue: Brain. Species: Rat. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:1000.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Rabbit Anti-HO-1 Polyclonal Antibody . Tissue: Brain. Species: Rat. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:1000.)
Related Product Information for anti-Hmox1 antibody
Background Info: Detects a ~33kDa protein corresponding to the molecular weight of native rat HO-1
Scientific Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
Scientific Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
Product Categories/Family for anti-Hmox1 antibody
References
1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411-419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925-10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759-1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129-135.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,006 Da
NCBI Official Full Name
heme oxygenase 1
NCBI Official Synonym Full Names
heme oxygenase (decycling) 1
NCBI Official Symbol
Hmox1
NCBI Official Synonym Symbols
Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32
NCBI Protein Information
heme oxygenase 1; heme oxygenase (decyclizing) 1
UniProt Protein Name
Heme oxygenase 1
UniProt Gene Name
Hmox1
UniProt Synonym Gene Names
HO-1
UniProt Entry Name
HMOX1_RAT
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Product Notes
The Hmox1 hmox1 (Catalog #AAA103730) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HO-1 Antibody reacts with Rat, Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's HO-1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Hmox1 hmox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HO-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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