Rabbit anti-Human HP1 Gamma/CBX3 Polyclonal Antibody | anti-CBX3 antibody

Anti-HP1 Gamma/CBX3 Antibody, Rabbit Polyclonal

Cat. Num. AAA259397

• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: HP1 Gamma/CBX3, Antibody; Anti-HP1 Gamma/CBX3 Antibody, Rabbit Polyclonal; HP1 Gamma/CBX3 Antibody, Rabbit PAb, Antigen Affinity Purified; chromobox homolog 3; Anti-HECH Antibody; Anti-HP1-GAMMA Antibody; Anti-HP1gamma Antibody; Anti-HP1Hs-gamma Antibody; anti-CBX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human HP1 Gamma/CBX3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CBX3 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the center region of the Human HP1 Gamma/CBX3
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human HP1 Gamma/CBX3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

IP (Immunoprecipitation)

(CBX3 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-CBX3 rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CBX3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size :21 kDa)

WB (Western Blot)

(Anti-CBX3 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa)

Related Product Information for anti-CBX3 antibody

CBX3was overexpressed in human PAAD tissues, which was associated with poor prognosis of overall and disease-free survival of the patients. Overexpression ofCBX3induced the in vitro proliferation, anchorage-free growth, migration and invasion of the PAAD cells, and led to in vivo growth of orthotoptic PAAD tumors in mice. GO and KEGG pathway analysis, as well as experimental observation showed thatCBX3may be associated with cell cycle transition of PAAD cells, and cyclin-dependent kinase 1 (CDK1) and proliferating cell nuclear antigen (PCNA) may mediate the tumor-promoting action ofCBX3. CDK1 knockdown attenuated the cell cycle transition, proliferation and invasion ofCBX3-overexpressing PAAD cells. CBX3 in PAAD to be targeted by novel therapeutic strategies. Chromobox protein homolog 3(CBX3) has an important role in carcinogenesis by regulating several mechanisms, such as heterochromatin formation, gene silencing, DNA replication and repair. Silencing CBX3 in pancreatic cancer cells inhibited aerobic glycolysis, the basis for providing cancer cells with building blocks for macromolecule synthesis and ATP that required. CBX3 serves as a positive regulator of aerobic glycolysis via suppressing of the FBP1 in pancreatic cancer cells. Disrupting the CBX3-FBP1 signaling axis would be effective to treat pancreatic cancer and prevent aerobic glycolysis.