FCM/FACS (Flow Cytometry)
(HPRT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit HPRT1 Polyclonal Antibody | anti-HPRT1 antibody
HPRT1 Antibody (N-term)
Gene Names
HPRT1; HPRT; HGPRT
Reactivity
Human (Predicted Reactivity: Hamster, Monkey, Mouse, Pig, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HPRT1, Antibody; HPRT1 Antibody (N-term); Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase; HPRT1; HPRT; anti-HPRT1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Hamster, Monkey, Mouse, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HPRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids from the N-terminal region of human HPRT1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
65-94
Applicable Applications for anti-HPRT1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Converts guanine to guanosinemonophosphate, and hypoxanthine to inosinemonophosphate. Transfers the5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto thepurine. Plays a central role in the generation of purine nucleotides through the purinesalvage pathway.
Cellular Location
Cytoplasm.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HPRT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HPRT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(HPRT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HPRT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(HPRT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HPRT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene.)
WB (Western Blot)
(Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene.)
Related Product Information for anti-HPRT1 antibody
The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Product Categories/Family for anti-HPRT1 antibody
References
Ceballos-Picot,I., Hum. Mol. Genet. 18 (13), 2317-2327 (2009)
Sculley,D.G., Hum. Genet. 90 (3), 195-207 (1992)
Sculley,D.G., Hum. Genet. 90 (3), 195-207 (1992)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24579
NCBI Official Full Name
hypoxanthine-guanine phosphoribosyltransferase
NCBI Official Synonym Full Names
hypoxanthine phosphoribosyltransferase 1
NCBI Official Symbol
HPRT1
NCBI Official Synonym Symbols
HPRT; HGPRT
NCBI Protein Information
hypoxanthine-guanine phosphoribosyltransferase
UniProt Protein Name
Hypoxanthine-guanine phosphoribosyltransferase
UniProt Gene Name
HPRT1
UniProt Synonym Gene Names
HPRT; HGPRT; HGPRTase
UniProt Entry Name
HPRT_HUMAN
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Product Notes
The HPRT1 hprt1 (Catalog #AAA287347) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 65-94. The HPRT1 Antibody (N-term) reacts with Human (Predicted Reactivity: Hamster, Monkey, Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's HPRT1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HPRT1 hprt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HPRT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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