WB (Western Blot)
(WB: The recombinant protein E7-GST was separated in 10% SDS-PAGE, transferred onto Nitrocellulose membrane, and immunoblotted by Rabbit anti-HPV16/E7 (IN) (Cat#AAA14076) at 1:500. An immune-reactive band is observed at molecular weight ~50 kDa corresponding to the fusion protein size.)
Rabbit HPV Polyclonal Antibody | anti-HPV antibody
Rabbit anti HPV (IN) (Human Papillomavirus), E7 protein Polyclonal Antibody
Reactivity
HPV
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Epitope Affinity Purification
Synonyms
HPV, Antibody; Rabbit anti HPV (IN) (Human Papillomavirus), E7 protein Polyclonal Antibody; Protein E7; Human papillomavirus type 16; HPV16; anti-HPV antibody
Host
Rabbit
Reactivity
HPV
Clonality
Polyclonal
Specificity
This antibody recognizes ~40 kDa of HPV16/E7-GST recombinant protein. The other species are not tested.
Purity/Purification
Purified by Epitope Affinity Purification
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-HPV antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Suggested Working Dilutions -
ELISA: 0.01-0.1 ug/ml
Immunohistochemistry: 2-10 ug/ml
Western Blot: 0.1-1 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
ELISA: 0.01-0.1 ug/ml
Immunohistochemistry: 2-10 ug/ml
Western Blot: 0.1-1 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Preparation
A synthetic peptide VQSTHVDIRTLEDLLMGTLGVCSQKP corresponding to the internal sequence of human papillomavirus type 16.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C - 8°C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
WB (Western Blot)
(WB: The recombinant protein E7-GST was separated in 10% SDS-PAGE, transferred onto Nitrocellulose membrane, and immunoblotted by Rabbit anti-HPV16/E7 (IN) (Cat#AAA14076) at 1:500. An immune-reactive band is observed at molecular weight ~50 kDa corresponding to the fusion protein size.)
WB (Western Blot)
(WB: The recombinant protein E7-GST was separated in 10% SDS-PAGE, transferred onto Nitrocellulose membrane, and immunoblotted by Rabbit anti-HPV16/E7 (IN) (Cat#AAA14076) at 1:500. An immune-reactive band is observed at molecular weight ~50 kDa corresponding to the fusion protein size.)
Related Product Information for anti-HPV antibody
The strain of Human papilloma virus 16 (HPV16 or E7 protein) is directly linked to cervical cancer and can be identified with a colposcopy and biopsy. Human papilloma virus is one of the most common causes of sexually transmitted infection in the world. E7 protein has transforming and trans-activating activities that are known to disrupt the function of host retinoblastoma protein (Rb1). E6 is a transcriptional trans-activator with transforming activities targeting p53 tumor suppressor. HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans. This antibody was raised in rabbit against protein E7 of human papillomavirus type 16 (HPV16) which is mainly used for research on sexually-transmitted diseases and gynecology tumor.
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Product Notes
The HPV (Catalog #AAA14076) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti HPV (IN) (Human Papillomavirus), E7 protein Polyclonal Antibody reacts with HPV and may cross-react with other species as described in the data sheet. AAA Biotech's HPV can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). Suggested Working Dilutions - ELISA: 0.01-0.1 ug/ml Immunohistochemistry: 2-10 ug/ml Western Blot: 0.1-1 ug/ml Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the HPV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HPV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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