FCM/FACS (Flow Cytometry)
(HRASLS3 Antibody (C-term) (AAA289272) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse HRASLS3 Polyclonal Antibody | anti-PLA2G16 antibody
HRASLS3 Antibody (C-term)
Gene Names
PLA2G16; AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; H-REV107-1
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HRASLS3, Antibody; HRASLS3 Antibody (C-term); HRAS-like suppressor 3; HRSL3; Adipose-specific phospholipase A2; AdPLA; Group XVI phospholipase A1/A2; H-rev 107 protein homolog; HRAS-like suppressor 1; HREV107-1; HREV107-3; Renal carcinoma antigen NY-REN-65; PLA2G16; HRASLS3; HREV107; anti-PLA2G16 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HRASLS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-132 amino acids from the C-terminal region of human HRASLS3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
104-132
Sequence Length
162
Applicable Applications for anti-PLA2G16 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Function
Lipid-modifying enzyme that acts as major regulator of adipocyte lipolysis by catalyzing the release of fatty acids from phospholipidsin adipose tissue (PubMed: , PubMed: 19047760, PubMed: , PubMed: 22605381, PubMed: ). Shows phospholipase A1 and A2 activity, catalyzingthe calcium- independent hydrolysis of acylgroups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE) (PubMed: , PubMed: 19047760, PubMed: , PubMed: 22605381, PubMed: ). For mostsubstrates, phospholipase A1 activity is much higher than phospholipase A2 activity (PubMed: ). Phospholipase activity causes decreased in tracellular levels of ether-type lipids,affecting peroxisome metabolism (By similarity). May also have acyltransferase activity: catalyzes both N-acylation of phosphatidylethanolamine to form N-acyl-phosphatidylethanolamine and O-acylation of lyso-phosphatidylcholines to form phosphatidylcholines (PubMed: , PubMed: 25383759). The relevance of acyltransferase activity in vivo is however unclear and would require additional evidences (PubMed: , PubMed: 25383759). Also has weak lysophospholipase activity (By similarity).
Cellular Location
Membrane; Single-pass membrane protein. Cytoplasm. Cytoplasm, perinuclear region{ECO:0000250|UniProtKB:Q8R3U1}.Peroxisome membrane{ECO:0000250|UniProtKB:Q8R3U1}
Tissue Location
Widely expressed. low expression, if any, inhematopoietic cells and thymus. In testis,confined to round spermatids. Expressed in normal ovarian epithelial cells. Down-regulated in some ovarian carcinomas and testicular germ cell tumors. Highly expressedin white adipose tissue (PubMed:19136964).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HRASLS3 Antibody (C-term) (AAA289272) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HRASLS3 Antibody (C-term) (AAA289272) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(HRASLS3 Antibody (C-term) (AAA289272) western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the HRASLS3 antibody detected HRASLS3 protein (arrow).)
WB (Western Blot)
(HRASLS3 Antibody (C-term) (AAA289272) western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the HRASLS3 antibody detected HRASLS3 protein (arrow).)
Related Product Information for anti-PLA2G16 antibody
PLA2G16 specifically catalyzes the release of fatty acids from phospholipids in adipose tissue. It also has a weak lysophospholipase activity (By similarity). Tumor suppressor that may be involved in interferon-dependent cell death.
Product Categories/Family for anti-PLA2G16 antibody
References
Uyama, T., et al. Biochim. Biophys. Acta 1791(12):1114-1124(2009)
Duncan, R.E., et al. J. Biol. Chem. 283(37):25428-25436(2008)
Nazarenko, I., et al. J. Cell. Sci. 120 (PT 8), 1393-1404 (2007)
Nazarenko, I., et al. Am. J. Pathol. 169(4):1427-1439(2006)
Duncan, R.E., et al. J. Biol. Chem. 283(37):25428-25436(2008)
Nazarenko, I., et al. J. Cell. Sci. 120 (PT 8), 1393-1404 (2007)
Nazarenko, I., et al. Am. J. Pathol. 169(4):1427-1439(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17937
NCBI Official Full Name
HRAS-like suppressor 3
NCBI Official Synonym Full Names
phospholipase A2, group XVI
NCBI Official Symbol
PLA2G16
NCBI Official Synonym Symbols
AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; H-REV107-1
NCBI Protein Information
HRAS-like suppressor 3
UniProt Protein Name
HRAS-like suppressor 3
UniProt Gene Name
PLA2G16
UniProt Synonym Gene Names
HRASLS3; HREV107; HRSL3; AdPLA
UniProt Entry Name
HRSL3_HUMAN
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Product Notes
The PLA2G16 pla2g16 (Catalog #AAA289272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 104-132. The HRASLS3 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HRASLS3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PLA2G16 pla2g16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRASLS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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