Rabbit anti-Human HRI Polyclonal Antibody | anti-EIF2AK1 antibody

HRI Antibody (N-term)

Cat. Num. AAA28727

• Gene Names: EIF2AK1; HCR; HRI
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: HRI, Antibody; HRI Antibody (N-term); Eukaryotic translation initiation factor 2-alpha kinase 1, Heme-controlled repressor, HCR, Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Heme-regulated inhibitor, Hemin-sensitive initiation factor 2-alpha kinase, EIF2AK1, HRI, KIAA1369 ; anti-EIF2AK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This HRI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HRI.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide; prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 1-30
• Sequence Length: 629

• Applicable Applications for anti-EIF2AK1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100
• Function: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions (PubMed:32132706, PubMed:32132707). Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as heme deficiency, oxidative stress, osmotic shock, mitochondrial dysfunction and heat shock (PubMed:32132706, PubMed:32132707). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming (PubMed:32132706, PubMed:32132707). Acts as a key sensor of heme-deficiency: in normal conditions, binds hemin via a cysteine thiolate and histidine nitrogenous coordination, leading to inhibit the protein kinase activity (By similarity). This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell: heme depletion relieves inhibition and stimulates kinase activity, activating the ISR (By similarity). Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions (By similarity). In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties (By similarity). It thereby plays an essential protective role for RBC survival in anemias of iron deficiency (By similarity). Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes (By similarity). May also regulate endoplasmic reticulum (ER) stress during acute heme- deficient conditions (By similarity). Also activates the ISR in response to mitochondrial dysfunction: HRI/EIF2AK1 protein kinase activity is activated upon binding to the processed form of DELE1 (S- DELE1), thereby promoting the ATF4-mediated reprogramming (PubMed:32132706, PubMed:32132707).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Western blot analysis of anti-HRI Pab AAA28727 in A375 cell lysate. HRI (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-EIF2AK1 antibody

Background: The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme.

Product Categories/Family for anti-EIF2AK1 antibody

Signal Transduction

References

Rafie-Kolpin, M., et al., Biochemistry 42(21):6536-6544 (2003).
Chen, J.J., et al., Trends Biochem. Sci. 20(3):105-108 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 197245437
• NCBI GeneID: 27102
• NCBI Accession #: NP_001127807.1; NP_055228.2
• NCBI GenBank Nucleotide #: NM_001134335.1
• UniProt Accession #: Q9BQI3; Q549K6; Q8NBW3; Q9HC02; Q9NYE0; Q9P0V6; Q9P1J5; Q9P2H8; Q9UHG4; A8K2R2
• Molecular Weight: Calculated MW: 71106
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 1 isoform b
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 1
• NCBI Official Symbol: EIF2AK1
• NCBI Official Synonym Symbols: HCR; HRI
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 1
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 1
• UniProt Gene Name: EIF2AK1
• UniProt Synonym Gene Names: HRI; KIAA1369; HCR
• UniProt Entry Name: E2AK1_HUMAN