FCM/FACS (Flow Cytometry)
(HRIHFB2025 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human HRIHFB2025 Polyclonal Antibody | anti-CBY1 antibody
HRIHFB2025 Antibody (C-term)
Gene Names
CBY1; CBY; arb1; PGEA1; C22orf2; PIGEA14; PIGEA-14; HS508I15A
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HRIHFB2025, Antibody; HRIHFB2025 Antibody (C-term); Protein chibby homolog 1; ARPP-binding protein; Cytosolic leucine-rich protein; PIGEA-14; PKD2 interactor; Golgi and endoplasmic reticulum-associated 1; CBY1; ARB1; C22orf2; CBY; PGEA1; anti-CBY1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HRIHFB2025 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the C-terminal region of human HRIHFB2025.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
98-126
Sequence Length
169
Applicable Applications for anti-CBY1 antibody
ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Cellular Location
Nucleus speckle. Golgi apparatus, trans- Golgi network. Note=Nuclear, in a punctate manner. Also found in the trans-Golgi
Tissue Location
Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors
Function
Inhibits the Wnt/Wingless pathway by binding to CTNNB1/beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HRIHFB2025 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HRIHFB2025 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with HRIHFB2025 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with HRIHFB2025 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of HRIHFB2025 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). HRIHFB2025 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of HRIHFB2025 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). HRIHFB2025 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CBY1 antibody
Inhibits the Wnt/Wingless pathway by binding to beta catenin and inhibiting beta catenin mediated transcriptional activation by competing with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin 2/PKD2 and possibly of other intracellular proteins.
Product Categories/Family for anti-CBY1 antibody
References
Gauci S., et.al., Anal. Chem. 81:4493-4501(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein chibby homolog 1 isoform b
NCBI Official Synonym Full Names
chibby homolog 1 (Drosophila)
NCBI Official Symbol
CBY1
NCBI Official Synonym Symbols
CBY; arb1; PGEA1; C22orf2; PIGEA14; PIGEA-14; HS508I15A
NCBI Protein Information
protein chibby homolog 1
UniProt Protein Name
Protein chibby homolog 1
UniProt Gene Name
CBY1
UniProt Synonym Gene Names
ARB1; C22orf2; CBY; PGEA1
UniProt Entry Name
CBY1_HUMAN
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Product Notes
The CBY1 cby1 (Catalog #AAA286340) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 98-126. The HRIHFB2025 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HRIHFB2025 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CBY1 cby1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRIHFB2025, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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