HRP Conjugated Goat Anti-mouse IgM Secondary Antibody
HRP Conjugated Goat Anti-mouse IgM (u-chain specific) secondary antibody
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
The antibody was purified from antiserum by immunoaffinity chromatography.
Synonyms
HRP Conjugated Goat Anti-mouse IgM, Secondary Antibody; HRP Conjugated Goat Anti-mouse IgM (u-chain specific) secondary antibody; HRP Conjugated Goat Anti-mouse IgM secondary antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgM
Specificity
This peroxidase conjugated antibody is specific for mouse IgM and shows no cross-reactivity with mouse IgG.
Purity/Purification
The antibody was purified from antiserum by immunoaffinity chromatography.
Form/Format
Concentrated, Liquid; 0.25 mg of peroxidase conjugated specific antibody, 0.01 M PBS (pH7.4), 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for HRP Conjugated Goat Anti-mouse IgM secondary antibody
ELISA, WB (Western Blot)
Immunogen
Mouse IgM (whole molecular).
Labeling Method
Goat anti-mouse IgM is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK. (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)
Preparation of Diluent Buffer
Use 0.01M TBS or 0.01M PBS to dilute. See “Recommended Dilutions” below for details.
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation and Storage
Store at -20°C for one year from date of receipt. Avoid repeated freezing and thawing.
Related Product Information for HRP Conjugated Goat Anti-mouse IgM secondary antibody
This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgM.
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Product Notes
The HRP Conjugated Goat Anti-mouse IgM (Catalog #AAA45603) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The HRP Conjugated Goat Anti-mouse IgM (u-chain specific) secondary antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HRP Conjugated Goat Anti-mouse IgM can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HRP Conjugated Goat Anti-mouse IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRP Conjugated Goat Anti-mouse IgM, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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