IF (Immunofluorescence)
(AAA322542 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit HRT2 Polyclonal Antibody | anti-HRT2 antibody
HRT2 Antibody
Gene Names
HEY2; GRL; CHF1; HRT2; HERP1; HESR2; bHLHb32; GRIDLOCK
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HRT2, Antibody; HRT2 Antibody; bHLHb32; Cardiovascular helix loop helix factor 1; Cardiovascular helix-loop-helix factor 1; CHF1; Class B basic helix-loop-helix protein 32; GRIDLOCK; GRL; Hairy and enhancer of split related 2; Hairy and enhancer of split-related protein 2; Hairy related transcription factor 2; Hairy-related transcription factor 2; Hairy/enhancer-of-split related with YRPW motif protein 2; hCHF1; HERP; HERP1; HES related repressor protein 1; HES-related repressor protein 2; HESR-2; HESR2; hey2; HEY2_HUMAN; hHRT2; HRT-2; HRT2; MGC10720; Protein gridlock homolog; anti-HRT2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HRT2 antibody detects endogenous levels of total HRT2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
337
Applicable Applications for anti-HRT2 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human HRT2
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA322542 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA322542 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of HRT2 using K562 whole cell lysates)
WB (Western Blot)
(Western blot analysis of HRT2 using K562 whole cell lysates)
Related Product Information for anti-HRT2 antibody
Function: Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Subunit Structure: May self-associate (By similarity). Interacts with GATA4, HES1 and HEYL (By similarity). Interacts with HDAC1, NCOR1 and SIN3A (By similarity). Interacts with ARNT and GATA6.
Similarity: Belongs to the HEY family.
Subunit Structure: May self-associate (By similarity). Interacts with GATA4, HES1 and HEYL (By similarity). Interacts with HDAC1, NCOR1 and SIN3A (By similarity). Interacts with ARNT and GATA6.
Similarity: Belongs to the HEY family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 36 kDa
Predicted: 36 kDa
Predicted: 36 kDa
NCBI Official Full Name
hairy/enhancer-of-split related with YRPW motif protein 2
NCBI Official Synonym Full Names
hes related family bHLH transcription factor with YRPW motif 2
NCBI Official Symbol
HEY2
NCBI Official Synonym Symbols
GRL; CHF1; HRT2; HERP1; HESR2; bHLHb32; GRIDLOCK
NCBI Protein Information
hairy/enhancer-of-split related with YRPW motif protein 2
UniProt Protein Name
Hairy/enhancer-of-split related with YRPW motif protein 2
UniProt Gene Name
HEY2
UniProt Synonym Gene Names
BHLHB32; CHF1; GRL; HERP; HERP1; HRT2; hCHF1; bHLHb32; HESR-2; HRT-2; hHRT2
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Product Notes
The HRT2 hey2 (Catalog #AAA322542) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HRT2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HRT2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the HRT2 hey2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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