WB (Western Blot)
(Detection of human hSET1 by western blot. Samples: Nuclear extract (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-hSET1 antibody AAA210865 (lot AAA210865-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human hSET1 Polyclonal Antibody | anti-hSET1 antibody
Rabbit anti-hSET1 Antibody, Affinity Purified
Gene Names
SETD1A; Set1; KMT2F; Set1A
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
hSET1, Antibody; Rabbit anti-hSET1 Antibody, Affinity Purified; Set1A; histone-lysine N-methyltransferase SETD1A; hSET1A; KMT2F; lysine N-methyltransferase 2F; SET domain-containing protein 1A; Set1; set1/Ash2 histone methyltransferase complex subunit SET1; Histone-lysine N-methyltransferase SETD1A; SET domain containing 1A; anti-hSET1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-hSET1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Bovine, Dog, Horse, Pig, Panda, Orangutan, Monkey, Gorilla, Chimpanzee
Immunogen
Between 1657 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human hSET1 by western blot. Samples: Nuclear extract (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-hSET1 antibody AAA210865 (lot AAA210865-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human hSET1 by western blot. Samples: Nuclear extract (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-hSET1 antibody AAA210865 (lot AAA210865-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human hSET1 by western blot of immunoprecipitates. Samples: Nuclear extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hSET1 antibody AAA210865 (lot AAA210865-2) used for IP at 6 ug per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody AAA210865-1. For blotting immunoprecipitated hSET1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human hSET1 by western blot of immunoprecipitates. Samples: Nuclear extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hSET1 antibody AAA210865 (lot AAA210865-2) used for IP at 6 ug per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody AAA210865-1. For blotting immunoprecipitated hSET1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-hSET1 antibody
hSET1 is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. The Set class of proteins takes its name from the 130-140 aa motif SET domain identified in drosophila proteins. hSET1 associates with a multitude of proteins in a Set1-like complex which includes hSet1, Ash2L, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, and HCF1.
Product Categories/Family for anti-hSET1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
186,034 Da
NCBI Official Full Name
histone-lysine N-methyltransferase SETD1A
NCBI Official Synonym Full Names
SET domain containing 1A
NCBI Official Symbol
SETD1A
NCBI Official Synonym Symbols
Set1; KMT2F; Set1A
NCBI Protein Information
histone-lysine N-methyltransferase SETD1A; hSET1A; lysine N-methyltransferase 2F; SET domain-containing protein 1A; set1/Ash2 histone methyltransferase complex subunit SET1
UniProt Protein Name
Histone-lysine N-methyltransferase SETD1A
UniProt Gene Name
SETD1A
UniProt Synonym Gene Names
; hSET1A
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Product Notes
The hSET1 setd1a (Catalog #AAA210865) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-hSET1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's hSET1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the hSET1 setd1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hSET1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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