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product-image-AAA179742_IF8.jpg IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HSP60 Polyclonal Antibody at dilution of 1:100.)

Rabbit HSP-60 Polyclonal Antibody | anti-HSP-60 antibody

HSP-60 Polyclonal Antibody

Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purification
Synonyms
HSP-60, Antibody; HSP-60 Polyclonal Antibody; 60 kDa heat shock protein; 60-KD; CH60; fa04a05; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 1 (chaperonin); Heat shock protein 60; Heat shock protein 65; heat shock protein family D (Hsp60) member 1; Hsp 60; HSP 65; mitochondrial; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; HLD; SPG; CPN; Chaperonin; HSPD; HuCHA; GROEL; HLD4; HSP-60; HSP65; HuCHA60; SPG13; CPN60; Mitochond; 60 kDa chaperonin; Chaperonin 60; HSP60; HSPD1; anti-HSP-60 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-HSP-60 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant Human HSP-60 protein expressed by E coli
Preparation and Storage
Store at -20 degree C Valid for 12 months. Avoid freeze/thaw cycles. Protected from prolonged exposure to light.

IF (Immunofluorescence)

(Immunofluorescence analysis of C6 cells using HSP60 Polyclonal Antibody at dilution of 1:100.)

product-image-AAA179742_IF8.jpg IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HSP60 Polyclonal Antibody at dilution of 1:100.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using HSP60 Polyclonal Antibody at dilution of 1:100.)

product-image-AAA179742_IF10.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using HSP60 Polyclonal Antibody at dilution of 1:100.)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells using HSP60 Polyclonal Antibody at dilution of 1:100.)

product-image-AAA179742_IF11.jpg IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using HSP60 Polyclonal Antibody at dilution of 1:100.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded Human liver using HSP60 Polyclonal Antibody at dilution of 1:1800.)

product-image-AAA179742_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver using HSP60 Polyclonal Antibody at dilution of 1:1800.)

WB (Western Blot)

(Western blot with Anti HSP-60 Polyclonal antibody at dilution of 1:1000. Lane 1: HeLa cell lysate, Lane 2: Mouse heart cell lysate, Lane 3: Rat brain tissue lysate, Lane 4: Rat heart tissue lysate, Lane 5: Rat spleen cell lysate, Lane 6: Rat liver tissue lysate.)

product-image-AAA179742_WB15.jpg WB (Western Blot) (Western blot with Anti HSP-60 Polyclonal antibody at dilution of 1:1000. Lane 1: HeLa cell lysate, Lane 2: Mouse heart cell lysate, Lane 3: Rat brain tissue lysate, Lane 4: Rat heart tissue lysate, Lane 5: Rat spleen cell lysate, Lane 6: Rat liver tissue lysate.)
Related Product Information for anti-HSP-60 antibody
Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically upregulated in response to cellular stress. Heat Shock Protein 60 (HSP60), also known as Chaperonin 60 (CPN60), is a mitochondrial matrix protein belonging to a highly conserved family of molecular chaperone and stress response proteins. HSP60 plays a role in stabilizing and refolding proteins in response to heat-shock or other cellular stress. Full length human HSP60 is 98% identical to mouse and rat HSP60.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,055 Da
NCBI Official Full Name
60 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock 60kDa protein 1 (chaperonin)
NCBI Official Symbol
HSPD1
NCBI Official Synonym Symbols
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
NCBI Protein Information
60 kDa heat shock protein, mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
UniProt Protein Name
60 kDa heat shock protein, mitochondrial
UniProt Gene Name
HSPD1
UniProt Synonym Gene Names
HSP60; CPN60; HSP-60; Hsp60
UniProt Entry Name
CH60_HUMAN

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Product Notes

The HSP-60 hspd1 (Catalog #AAA179742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSP-60 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSP-60 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HSP-60 hspd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP-60, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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