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product-image-AAA45487_IHC10.jpg IHC (Immunohistochemistry) (Anti-Hsp70 antibody, PA1813, IHC(F)IHC(F):Rat Intestine Tissue)

Rabbit anti-Human, Rat Hsp70 Polyclonal Antibody | anti-HSPA1A antibody

Anti-Hsp70 antibody

Gene Names
HSPA1B; HSP70-2; HSP70-1B
Reactivity
Human, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Hsp70, Antibody; Anti-Hsp70 antibody; Heat shock 70 kDa protein 1A/1B; heat shock 70kDa protein 1A; DAQB 147D11.1 001 antibody; FLJ54303 antibody; FLJ54370 antibody; FLJ54392 antibody; FLJ54408 antibody; FLJ75127 antibody; Heat shock 70 kDa protein 1 antibody; Heat shock 70 kDa protein 1/2 antibody; Heat shock 70 kDa protein 1A/1B antibody; heat shock 70kDa protein 1A antibody; Heat shock 70kDa protein 1B antibody; Heat shock induced protein antibody; heat shock protein 70 antibody; HSP70 1 antibody; HSP70 2 antibody; HSP70-1/HSP70-2 antibody; HSP70-1A antibody; HSP70.1 antibody; HSP70.1/HSP70.2 antibody; HSP70I antibody; HSP71_HUMAN antibody; HSP72 antibody; HSPA1 antibody; HSPA1A antibody; HSPA1B antibody; XXbac BCX40G17.3 001 antibody; anti-HSPA1A antibody
Ordering
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Sequence Length
641
Applicable Applications for anti-HSPA1A antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70 (577-596aa VISWLDANTLAEKDEFEHKR), different from the related mouse sequence by four amino acids and from the related rat sequence by three amino acids.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.

Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(Anti-Hsp70 antibody, PA1813, IHC(F)IHC(F):Rat Intestine Tissue)

product-image-AAA45487_IHC10.jpg IHC (Immunohistochemistry) (Anti-Hsp70 antibody, PA1813, IHC(F)IHC(F):Rat Intestine Tissue)

ICC (Immunocytochemistry)

(Anti-Hsp70 antibody, ICCICC: A549 Cell)

product-image-AAA45487_ICC11.jpg ICC (Immunocytochemistry) (Anti-Hsp70 antibody, ICCICC: A549 Cell)

IHC (Immunohiostchemistry)

(Anti-Hsp70 antibody, PA1813, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

product-image-AAA45487_IHC13.jpg IHC (Immunohiostchemistry) (Anti-Hsp70 antibody, PA1813, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

WB (Western Blot)

(Anti-Hsp70 antibody, Western blottingAll lanes: Anti Hsp70 at 0.5ug/mlLane 1: 293T Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: A431 Whole Cell Lysate at 40ugLane 5: PANC Whole Cell Lysate at 40ugPredicted bind size: 70KDObserved bind size: 70KD)

product-image-AAA45487_WB15.jpg WB (Western Blot) (Anti-Hsp70 antibody, Western blottingAll lanes: Anti Hsp70 at 0.5ug/mlLane 1: 293T Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: A431 Whole Cell Lysate at 40ugLane 5: PANC Whole Cell Lysate at 40ugPredicted bind size: 70KDObserved bind size: 70KD)
Related Product Information for anti-HSPA1A antibody
Description: Rabbit IgG polyclonal antibody for Heat shock 70 kDa protein 1A/1B(HSPA1A) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Rat.
Background: HSPA1(heat shock 70kDa protein 1A) also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. The HSPA1 gene is mapped on 6p21.33. Shimizu et al.(1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Heat shock 70 kDa protein 1A/1B
NCBI Official Synonym Full Names
heat shock 70kDa protein 1B
NCBI Official Symbol
HSPA1B
NCBI Official Synonym Symbols
HSP70-2; HSP70-1B
NCBI Protein Information
heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; heat shock 70kD protein 1B; heat shock 70 kDa protein 1/2
UniProt Protein Name
Heat shock 70 kDa protein 1A/1B
UniProt Gene Name
HSPA1A
UniProt Synonym Gene Names
HSPA1; HSX70; HSP70-1/HSP70-2; HSP70.1/HSP70.2
UniProt Entry Name
HSP71_HUMAN

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Product Notes

The HSPA1A hspa1a (Catalog #AAA45487) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hsp70 antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp70 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HSPA1A hspa1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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