IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)
Rabbit Hsp90 Polyclonal Antibody | anti-HSP90AB1 antibody
Hsp90 Antibody
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Rat, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, ELISA, Western Blot
Synonyms
Hsp90, Antibody; Hsp90 Antibody; Hsp84; Hsp86; Hsp90A; Hsp90AA1; Hsp90AB1; Hsp90B; HspC1; HspC2; HspCAL1; HspCAL4; Hsp90N; anti-HSP90AB1 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Form/Format
Rabbit Antiserum
Sequence Length
724
Applicable Applications for anti-HSP90AB1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot)
Certificate of Analysis
A 1:40000 dilution of SPC-104 was sufficient for detection of 0.2mg of purified hsp90 by ECL immunoblot analysis.
Conjugate
Unconjugated
Storage Buffer
Rabbit antiserum
Preparation and Storage
-20 degree C
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)
WB (Western Blot)
(Western blot analysis of Human Cell line lysates showing detection of HSP90 protein using Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104). Load: 2 ugHsp90beta. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104) at 1:10000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
WB (Western Blot)
(Western blot analysis of Human Cell line lysates showing detection of HSP90 protein using Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104). Load: 2 ugHsp90beta. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104) at 1:10000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Related Product Information for anti-HSP90AB1 antibody
Background Info: Detects ~90kDa proteins corresponding to the molecular mass of Hsp90alpha/beta.
Scientific Background: Hsp90 is a highly conserved and essential stress protein Hsp90 is a highly conserved and essential stress protein
Scientific Background: Hsp90 is a highly conserved and essential stress protein Hsp90 is a highly conserved and essential stress protein
Product Categories/Family for anti-HSP90AB1 antibody
References
1. Arlander S.J.H., et al. (2003) J Biol Chem 278: 52572-52577. 2. Pearl H., et al. (2001) Adv Protein Chem 59:157-186. 3. Neckers L., et al. (2002) Trends Mol Med 8:S55-S61. 4. Pratt W., Toft D. (2003) Exp Biol Med 228:111-133. 5. Pratt W., Toft D. (1997) Endocr Rev 18: 306-360. 6. Pratt W.B. (1998) Proc Soc Exptl Biol Med 217: 420-434. 7. Whitesell L., et al. (1994) Proc Natl Acad Sci USA 91: 8324- 8328.
1. Wagatsuma, A. et al. (2011). Pharmacological inhibition of HSP90 activity negatively modulates myogenic differentiation and cell survival in C2C12 cells. Mol and Cel Biochem. 358 (1-2), 265-280. doi: 10.1007/s11010-011-0977-0
2. Verheyen, A. et al. (2012). Systemic anti-vascular endothelial growth factor therapies induce a painful sensory neuropathy. Brain. 135 (9), 2629-2641. doi: 10.1093/brain/aws145
3. Zhang, S. et al. (2013). B Cell–Specific Deficiencies in mTOR Limit Humoral Immune Responses. Journal of Immunology. Published online. doi: 10.4049/jimmunol.1201767
1. Wagatsuma, A. et al. (2011). Pharmacological inhibition of HSP90 activity negatively modulates myogenic differentiation and cell survival in C2C12 cells. Mol and Cel Biochem. 358 (1-2), 265-280. doi: 10.1007/s11010-011-0977-0
2. Verheyen, A. et al. (2012). Systemic anti-vascular endothelial growth factor therapies induce a painful sensory neuropathy. Brain. 135 (9), 2629-2641. doi: 10.1093/brain/aws145
3. Zhang, S. et al. (2013). B Cell–Specific Deficiencies in mTOR Limit Humoral Immune Responses. Journal of Immunology. Published online. doi: 10.4049/jimmunol.1201767
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,264 Da
NCBI Official Full Name
heat shock protein HSP 90-beta isoform a
NCBI Official Synonym Full Names
heat shock protein 90kDa alpha (cytosolic), class B member 1
NCBI Official Symbol
HSP90AB1
NCBI Official Synonym Symbols
HSP84; HSPC2; HSPCB; D6S182; HSP90B
NCBI Protein Information
heat shock protein HSP 90-beta; HSP90-beta; heat shock 84 kDa; heat shock 90kD protein 1, beta
UniProt Protein Name
Heat shock protein HSP 90-beta
UniProt Gene Name
HSP90AB1
UniProt Synonym Gene Names
HSP90B; HSPC2; HSPCB; HSP 90; HSP 84; HSP84
UniProt Entry Name
HS90B_HUMAN
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Product Notes
The HSP90AB1 hsp90ab1 (Catalog #AAA103652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hsp90 Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp90 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HSP90AB1 hsp90ab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp90, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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