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WB (Western Blot)
(Western blot analysis of extracts from HeLa cells (Lane 2), CoLo cells (Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5), using HSP90B (Ab-254) antiobdy. The lane on the left is treated with synthesized peptide.)
Rabbit anti-Human HSP90B Polyclonal Antibody | anti-HSP90B antibody
HSP90B (Ab-254) Antibody
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
HSP90B, Antibody; HSP90B (Ab-254) Antibody; HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2; anti-HSP90B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total HSP90B protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
724
Applicable Applications for anti-HSP90B antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthesized non-phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-S(p)-D-E).
Target Name
HSP90B
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells (Lane 2), CoLo cells (Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5), using HSP90B (Ab-254) antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells (Lane 2), CoLo cells (Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5), using HSP90B (Ab-254) antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells treated with TNF-a (20ng/ml, 30min), using HSP90B (Ab-254) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells treated with TNF-a (20ng/ml, 30min), using HSP90B (Ab-254) antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (Ab-254) antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (Ab-254) antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (Ab-254) antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (Ab-254) antibody.)
Related Product Information for anti-HSP90B antibody
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.Lothar Goretzki, AACR Meeting Abstracts, Apr 2006; 2006: 468.Jean-Fran ois Louvion, Mol. Biol. Cell, Nov 1998; 9: 3071.Angelika Lueking, Mol. Cell. Proteomics, Dec 2003; 2: 1342.Jason Fontana Circ, Res., May 2002; 90: 866.
Product Categories/Family for anti-HSP90B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,264 Da
NCBI Official Full Name
heat shock protein HSP 90-beta isoform a
NCBI Official Synonym Full Names
heat shock protein 90kDa alpha (cytosolic), class B member 1
NCBI Official Symbol
HSP90AB1
NCBI Official Synonym Symbols
HSP84; HSPC2; HSPCB; D6S182; HSP90B
NCBI Protein Information
heat shock protein HSP 90-beta
UniProt Protein Name
Heat shock protein HSP 90-beta
UniProt Gene Name
HSP90AB1
UniProt Synonym Gene Names
HSP90B; HSPC2; HSPCB; HSP 90; HSP 84; HSP84
UniProt Entry Name
HS90B_HUMAN
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Product Notes
The HSP90B hsp90ab1 (Catalog #AAA303979) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSP90B (Ab-254) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSP90B can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HSP90B hsp90ab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP90B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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