IF (Immunofluorescence)
(Dilution: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Incubation: Anti-HSPA12A Antibody, conjugated (bs-18076R-FITC) 1:400, 1.5 hours at 37 degree C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.)
Rabbit HSPA12A Polyclonal Antibody | anti-HSPA12A antibody
HSPA12A Polyclonal Antibody
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HSPA12A, Antibody; HSPA12A Polyclonal Antibody; Heat shock 70 kDa protein 12A; HSPA12A; KIAA0417; anti-HSPA12A antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-HSPA12A antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Dilution: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Incubation: Anti-HSPA12A Antibody, conjugated (bs-18076R-FITC) 1:400, 1.5 hours at 37 degree C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.)
IF (Immunofluorescence)
(Dilution: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Incubation: Anti-HSPA12A Antibody, conjugated (bs-18076R-FITC) 1:400, 1.5 hours at 37 degree C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.)
WB (Western Blot)
(Sample: A673 Cell (Human) Lysate at 40 ugPrimary: Anti-HSPA12A (bs-18076R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 75 kDObserved band size: 73 kD)
WB (Western Blot)
(Sample: A673 Cell (Human) Lysate at 40 ugPrimary: Anti-HSPA12A (bs-18076R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 75 kDObserved band size: 73 kD)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (HSPA12A) Polyclonal Antibody, Unconjugated (bs-18076R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (HSPA12A) Polyclonal Antibody, Unconjugated (bs-18076R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75kDa
NCBI Official Full Name
heat shock 70 kDa protein 12A isoform 2
NCBI Official Synonym Full Names
heat shock protein family A (Hsp70) member 12A
NCBI Official Symbol
HSPA12A
NCBI Protein Information
heat shock 70 kDa protein 12A
UniProt Protein Name
Heat shock 70 kDa protein 12A
UniProt Gene Name
HSPA12A
UniProt Synonym Gene Names
KIAA0417
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Product Notes
The HSPA12A hspa12a (Catalog #AAA291769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPA12A Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA12A can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HSPA12A hspa12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPA12A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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