IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cell using HSPA1A antibody. Blue: DAPI for nuclear staining.)
Rabbit anti-Human HSPA1A Polyclonal Antibody | anti-HSPA1A antibody
HSPA1A Antibody
Gene Names
HSPA1B; HSP70-2; HSP70.2; HSP70-1B
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
HSPA1A, Antibody; HSPA1A Antibody; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; anti-HSPA1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total HSPA1A protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-HSPA1A antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
A synthetic peptide of human HSPA1A.
Target Name
HSPA1A
Preparation and Storage
Store at -20 degree C
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cell using HSPA1A antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cell using HSPA1A antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HSPA1A antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HSPA1A antibody.)
Related Product Information for anti-HSPA1A antibody
HSPA1A and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSPA1A and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner (1). HSPA1A has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSPA1A and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop, and p23 (2,3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1,4). When the ubiquitin ligase CHIP associates with the HSPA1A/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSPA1A/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2).
Product Categories/Family for anti-HSPA1A antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HSPA1A (Catalog #AAA307222) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPA1A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA1A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the HSPA1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPA1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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