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WB (Western Blot)
(Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.)
Rabbit HUWE1 / ARFBP1 Polyclonal Antibody | anti-HUWE1 antibody
Rabbit Polyclonal (IgG) to Human HUWE1 / ARFBP1
Gene Names
HUWE1; MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
HUWE1 / ARFBP1, Antibody; Rabbit Polyclonal (IgG) to Human HUWE1 / ARFBP1; Anti-HUWE1 / ARFBP1 Antibody (C-Terminus) IHC-plus; HUWE1; ARF-binding protein 1; ARF-BP1; BJ-HCC-24 tumor antigen; Ib772; KIAA0312; LASU1; Mcl-1 ubiquitin ligase E3; HECTH9; MULE; HSPC272; KIAA1578; URE-B1; UREB1; URE-binding protein 1; HECT domain protein LASU1; Large structure of UREB1; Human HUWE1; ARFBP1; anti-HUWE1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
4374
Applicable Applications for anti-HUWE1 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
A 19 amino acid peptide from near the carboxy terminus of human ARF-BP1.
Immunogen Type
Synthetic peptide
Immunogen
HUWE1 / ARFBP1 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1.
Antigen Modification
C-Terminus
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
WB (Western Blot)
(Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.)
WB (Western Blot)
(Western blot of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.)
ICC (Immunocytochemistry)
(Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.)
ICC (Immunocytochemistry)
(Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.)
IF (Immunofluorescence)
(Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.)
IF (Immunofluorescence)
(Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.)
IHC (Immunohiostchemistry)
(Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohiostchemistry)
(Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Related Product Information for anti-HUWE1 antibody
HUWE1 is a member of the HECT E3 ubiquitin ligase family. The HECT domain lies in the C-terminus and contains the active-site cysteine which forms an intermediate ubiquitin-thioester bond. E3 family members are divided into three subfamilies based on their protein-protein interaction domains; this gene encodes a member of the SI(ngle)-HECT E3 subfamily. In lung, breast, and colorectal carcinomas, this gene is highly expressed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
481,018 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase HUWE1
NCBI Official Synonym Full Names
HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
NCBI Official Symbol
HUWE1
NCBI Official Synonym Symbols
MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
NCBI Protein Information
E3 ubiquitin-protein ligase HUWE1; ARF-binding protein 1; URE-binding protein 1; BJ-HCC-24 tumor antigen; large structure of UREB1; HECT domain protein LASU1; Mcl-1 ubiquitin ligase E3; upstream regulatory element-binding protein 1; homologous to E6AP carboxyl terminus homologous protein 9
UniProt Protein Name
E3 ubiquitin-protein ligase HUWE1
UniProt Gene Name
HUWE1
UniProt Synonym Gene Names
KIAA0312; KIAA1578; UREB1; ARF-BP1; HectH9; LASU1; Mule; URE-B1; URE-binding protein 1
UniProt Entry Name
HUWE1_HUMAN
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Product Notes
The HUWE1 huwe1 (Catalog #AAA50784) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human HUWE1 / ARFBP1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HUWE1 / ARFBP1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the HUWE1 huwe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HUWE1 / ARFBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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