FCM/FACS (Flow Cytometry)
(HYAL2 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human HYAL2 Polyclonal Antibody | anti-HYAL2 antibody
HYAL2 Antibody (C-term)
Gene Names
HYAL2; LUCA2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HYAL2, Antibody; HYAL2 Antibody (C-term); Hyaluronidase-2; Hyal-2; Hyaluronoglucosaminidase-2; Lung carcinoma protein 2; LuCa-2; HYAL2; LUCA2; anti-HYAL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HYAL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-412 amino acids from the C-terminal region of human HYAL2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.46 (varies by lot)
Sequence Positions
385-412
Sequence Length
473
Applicable Applications for anti-HYAL2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HYAL2 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HYAL2 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(HYAL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HYAL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(HYAL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HYAL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(HYAL2 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HYAL2 antibody detected the HYAL2 protein (arrow).)
WB (Western Blot)
(HYAL2 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HYAL2 antibody detected the HYAL2 protein (arrow).)
Related Product Information for anti-HYAL2 antibody
This gene encodes a weak acid-active hyaluronidase. The
encoded protein is similar in structure to other more active
hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major
glycosaminoglycans of the extracellular matrix. Hyaluronan and
fragments of hyaluronan are thought to be involved in cell
proliferation, migration and differentiation. Although it was
previously thought to be a lysosomal hyaluronidase that is active
at a pH below 4, the encoded protein is likely a GPI-anchored cell
surface protein. This hyaluronidase serves as a receptor for the
oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of
several related genes in a region of chromosome 3p21.3 associated
with tumor suppression. This gene encodes two alternatively spliced
transcript variants which differ only in the 5' UTR.
encoded protein is similar in structure to other more active
hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major
glycosaminoglycans of the extracellular matrix. Hyaluronan and
fragments of hyaluronan are thought to be involved in cell
proliferation, migration and differentiation. Although it was
previously thought to be a lysosomal hyaluronidase that is active
at a pH below 4, the encoded protein is likely a GPI-anchored cell
surface protein. This hyaluronidase serves as a receptor for the
oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of
several related genes in a region of chromosome 3p21.3 associated
with tumor suppression. This gene encodes two alternatively spliced
transcript variants which differ only in the 5' UTR.
Product Categories/Family for anti-HYAL2 antibody
References
Monzon, M.E., et al. J. Biol. Chem. 285(34):26126-26134(2010)
Tzuman, Y.C., et al. Neoplasia 12(1):51-60(2010)
de la Motte, C., et al. Am. J. Pathol. 174(6):2254-2264(2009)
Nykopp, T.K., et al. BMC Cancer 9, 143 (2009) :
Miller, A.D., et al. Osteoarthr. Cartil. 14(12):1315-1317(2006)
Tzuman, Y.C., et al. Neoplasia 12(1):51-60(2010)
de la Motte, C., et al. Am. J. Pathol. 174(6):2254-2264(2009)
Nykopp, T.K., et al. BMC Cancer 9, 143 (2009) :
Miller, A.D., et al. Osteoarthr. Cartil. 14(12):1315-1317(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53860
NCBI Official Full Name
hyaluronidase-2
NCBI Official Synonym Full Names
hyaluronoglucosaminidase 2
NCBI Official Symbol
HYAL2
NCBI Official Synonym Symbols
LUCA2
NCBI Protein Information
hyaluronidase-2
UniProt Protein Name
Hyaluronidase-2
UniProt Gene Name
HYAL2
UniProt Synonym Gene Names
LUCA2; Hyal-2; LuCa-2
UniProt Entry Name
HYAL2_HUMAN
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Product Notes
The HYAL2 hyal2 (Catalog #AAA290361) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 385-412. The HYAL2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HYAL2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HYAL2 hyal2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HYAL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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