WB (Western Blot)
(Anti-ICAM2 rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:47 kDa)
Rabbit anti-Human ICAM-2 Polyclonal Antibody | anti-ICAM2 antibody
Anti-ICAM-2 Antibody, Rabbit Polyclonal
Gene Names
ICAM2; CD102
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
ICAM-2, Antibody; Anti-ICAM-2 Antibody, Rabbit Polyclonal; ICAM-2/CD102 Antibody, Rabbit PAb, Antigen Affinity Purified; intercellular adhesion molecule 2; Anti-CD102 Antibody; anti-ICAM2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ICAM-2/CD102
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ICAM2 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human ICAM-2/CD102 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human ICAM-2/CD102 (rh ICAM-2/CD102). ICAM-2/CD102 specific IgG was purified by Human ICAM-2/CD102 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-ICAM2 rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:47 kDa)
WB (Western Blot)
(Anti-ICAM2 rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:47 kDa)
IP (Immunoprecipitation)
(ICAM2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-ICAM2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-ICAM2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 31 kDa)
IP (Immunoprecipitation)
(ICAM2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-ICAM2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-ICAM2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 31 kDa)
Related Product Information for anti-ICAM2 antibody
Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As the second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocyte with other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity, and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relationship with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in the identification of trichinosis recent infections, and in monitoring the prognosis and response to treatment.
References
Weber KS, et al. (2004) Sialylation of ICAM-2 on platelets impairs adhesion of leukocytes via LFA-1 and DC-SIGN. Inflammation. 28(4): 177-88.Tanaka H, et al. (2004) ICAM-2 gene therapy for peritoneal dissemination of scirrhous gastric carcinoma. Clin Cancer Res. 10(14): 4885-92.Younis AI, et al. (2005) Intercellular adhesion molecule-2 (ICAM-2) in experimental trichinosis. J Egypt Soc Parasitol. 35(3): 1019-26.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,654 Da
NCBI Official Full Name
intercellular adhesion molecule 2
NCBI Official Synonym Full Names
intercellular adhesion molecule 2
NCBI Official Symbol
ICAM2
NCBI Official Synonym Symbols
CD102
NCBI Protein Information
intercellular adhesion molecule 2; ICAM-2
UniProt Protein Name
Intercellular adhesion molecule 2
UniProt Gene Name
ICAM2
UniProt Synonym Gene Names
ICAM-2
UniProt Entry Name
ICAM2_HUMAN
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Product Notes
The ICAM2 icam2 (Catalog #AAA254830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ICAM-2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ICAM-2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ICAM2 icam2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICAM-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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