WB (Western Blot)
(Detection of human ICAM-3 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-3 antibody AAA212627 (lot AAA212627-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human ICAM-3 Polyclonal Antibody | anti-ICAM-3 antibody
Rabbit anti-ICAM-3 Antibody, Affinity Purified
Gene Names
ICAM3; CD50; CDW50; ICAM-R
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ICAM-3, Antibody; Rabbit anti-ICAM-3 Antibody, Affinity Purified; CD antigen CD50; ICAM-R; ICAM-3; CD50; intercellular adhesion molecule 3; CDW50; Intercellular adhesion molecule 3; anti-ICAM-3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ICAM-3 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Chimpanzee
Immunogen
Between 497 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ICAM-3 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-3 antibody AAA212627 (lot AAA212627-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human ICAM-3 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-3 antibody AAA212627 (lot AAA212627-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ICAM-3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-3 antibody AAA212627 (lot AAA212627-1) used for IP at 6 ug per reaction. For blotting immunoprecipitated ICAM-3, AAA212627 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ICAM-3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-3 antibody AAA212627 (lot AAA212627-1) used for IP at 6 ug per reaction. For blotting immunoprecipitated ICAM-3, AAA212627 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human ICAM-3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma infiltrating cells. Antibody: Affinity purified rabbit anti-ICAM-3 (Cat. No. AAA212627 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ICAM-3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma infiltrating cells. Antibody: Affinity purified rabbit anti-ICAM-3 (Cat. No. AAA212627 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-ICAM-3 antibody
ICAM-3 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule [taken from NCBI Entrez Gene (Gene ID: 3385)].
Product Categories/Family for anti-ICAM-3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,541 Da
NCBI Official Full Name
intercellular adhesion molecule 3
NCBI Official Synonym Full Names
intercellular adhesion molecule 3
NCBI Official Symbol
ICAM3
NCBI Official Synonym Symbols
CD50; CDW50; ICAM-R
NCBI Protein Information
intercellular adhesion molecule 3; ICAM-3; intercellular adhesion molecule-3
UniProt Protein Name
Intercellular adhesion molecule 3
UniProt Gene Name
ICAM3
UniProt Synonym Gene Names
ICAM-3
UniProt Entry Name
ICAM3_HUMAN
Similar Products
Product Notes
The ICAM-3 icam3 (Catalog #AAA212627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ICAM-3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ICAM-3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ICAM-3 icam3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICAM-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.