FCM/FACS (Flow Cytometry)
(ICB1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human ICB1 Polyclonal Antibody | anti-THEMIS2 antibody
ICB1 Antibody (C-term)
Gene Names
THEMIS2; ICB-1; C1orf38
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
ICB1, Antibody; ICB1 Antibody (C-term); Protein THEMIS2; Induced by contact to basement membrane 1 protein; Protein ICB-1; Thymocyte-expressed molecule involved in selection protein 2; THEMIS2; C1orf38; ICB1; anti-THEMIS2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ICB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-641 amino acids from the C-terminal region of human ICB1.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
613-641
Sequence Length
447
Applicable Applications for anti-THEMIS2 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Function
Function May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production (PubMed:20644716). Determines the threshold for activation of B cells by low-affinity and low-avidity ligands via PLCG2 activation and its downstream pathways (By similarity).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ICB1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ICB1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of ICB1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). ICB1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ICB1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). ICB1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-THEMIS2 antibody
Involved in cell adhesion. There are 4 isoforms produced by alternative splicing.
References
Springwald, A., et al. Cancer Invest. 27(6):669-672(2009)
Treeck, O., et al. Cytokine 32 (3-4), 137-142 (2005)
Treeck, O., et al. Leuk. Res. 26(8):765-769(2002)
Treeck, O., et al. Cytokine 32 (3-4), 137-142 (2005)
Treeck, O., et al. Leuk. Res. 26(8):765-769(2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72049
NCBI Official Full Name
protein THEMIS2 isoform 2
NCBI Official Synonym Full Names
thymocyte selection associated family member 2
NCBI Official Symbol
THEMIS2
NCBI Official Synonym Symbols
ICB-1; C1orf38
NCBI Protein Information
protein THEMIS2
UniProt Protein Name
Protein THEMIS2
UniProt Gene Name
THEMIS2
UniProt Synonym Gene Names
C1orf38; ICB1; Protein ICB-1
UniProt Entry Name
THMS2_HUMAN
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Product Notes
The THEMIS2 themis2 (Catalog #AAA289912) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 613-641. The ICB1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ICB1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the THEMIS2 themis2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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