WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using IFIT5 antibody.)
Rabbit anti-Human IFIT5 Polyclonal Antibody | anti-IFIT5 antibody
IFIT5 Antibody
Gene Names
IFIT5; P58; RI58; ISG58
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IFIT5, Antibody; IFIT5 Antibody; FLJ53857; FLJ92678; IFIT-5; IFIT5; IFIT5_HUMAN; Interferon induced protein with tetratricopeptide repeats 5; Interferon-induced protein with tetratricopeptide repeats 5; Retinoic acid and interferon inducible 58 kDa protein; Retinoic acid and interferon inducible protein (58kD); Retinoic acid-and interferon-inducible 58 kDa protein; RI58; anti-IFIT5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
IFIT5 antibody detects endogenous levels of total IFIT5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
482
Applicable Applications for anti-IFIT5 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Plasma Membrane
Tissue Specificity
By interferons (IFNs).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (92%), Bovine (92%), Horse (83%), Sheep (92%), Rabbit (83%), Dog (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using IFIT5 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using IFIT5 antibody.)
IF (Immunofluorescence)
(AAA325347 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA325347 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-IFIT5 antibody
Function: Interferon-induced RNA-binding protein involved in the human innate immune response. Has a broad and adaptable RNA structure recognition important for RNA recognition specificity in antiviral defense. Binds precursor and processed tRNAs as well as poly-U-tailed tRNA fragments (PubMed:25092312, PubMed:23317505, PubMed:23774268). Specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner (PubMed:23334420). Also recognizes and selectively binds AT-rich dsDNA (PubMed:23774268). Additionally, as a mediator in innate immunity, regulates positively IKK-NFKB signaling by sinergizing the recruitment of IKK to MAP3K7 (PubMed:26334375).
Subunit Structure: Monomer (PubMed:23317505, PubMed:23334420, PubMed:23774268). Interacts with MAP3K7 and the components of the IKK core complex CHUK, IKBKB and IKBKG; the interaction synergizes the recruitment of IKK to MAP3K7 and enhances IKK phosphorylation (PubMed:26334375).
Similarity: RNA recognition is mediated by a convoluted intramolecular fold of the TPR repeats (TPR eddy), which scaffolds unique additional helices that form an RNA binding cleft (PubMed:23317505 and PubMed:23334420). Undergoes a conformational change upon RNA-binding: unliganded exists in a more open conformation, facilitating RNA entry (PubMed:23334420). Belongs to the IFIT family.
Subunit Structure: Monomer (PubMed:23317505, PubMed:23334420, PubMed:23774268). Interacts with MAP3K7 and the components of the IKK core complex CHUK, IKBKB and IKBKG; the interaction synergizes the recruitment of IKK to MAP3K7 and enhances IKK phosphorylation (PubMed:26334375).
Similarity: RNA recognition is mediated by a convoluted intramolecular fold of the TPR repeats (TPR eddy), which scaffolds unique additional helices that form an RNA binding cleft (PubMed:23317505 and PubMed:23334420). Undergoes a conformational change upon RNA-binding: unliganded exists in a more open conformation, facilitating RNA entry (PubMed:23334420). Belongs to the IFIT family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 50 kD
Predicted: 56 kDa
Predicted: 56 kDa
NCBI Official Full Name
interferon-induced protein with tetratricopeptide repeats 5
NCBI Official Synonym Full Names
interferon induced protein with tetratricopeptide repeats 5
NCBI Official Symbol
IFIT5
NCBI Official Synonym Symbols
P58; RI58; ISG58
NCBI Protein Information
interferon-induced protein with tetratricopeptide repeats 5
UniProt Protein Name
Interferon-induced protein with tetratricopeptide repeats 5
UniProt Gene Name
IFIT5
UniProt Synonym Gene Names
ISG58; RI58; IFIT-5; P58
Similar Products
Product Notes
The IFIT5 ifit5 (Catalog #AAA325347) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFIT5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IFIT5 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the IFIT5 ifit5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFIT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.