WB (Western Blot)
(Western blot analysis of extracts of HEK-293 cells, using IFT20 antibody.)
Rabbit IFT20 Polyclonal Antibody | anti-IFT20 antibody
IFT20 Antibody
Reactivity
Human, Mouse, Dog
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IFT20, Antibody; IFT20 Antibody; hIFT20; Intraflagellar transport 20 homolog (Chlamydomonas); Intraflagellar transport protein 20 like; Intraflagellar transport protein IFT20; anti-IFT20 antibody
Host
Rabbit
Reactivity
Human, Mouse, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
IFT20 antibody detects endogenous levels of IFT20.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
158
Applicable Applications for anti-IFT20 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human IFT20.
Subcellular Location
Golgi Apparatus. Cilium. Centrosome, Centriole.
Tissue Specificity
Expressed in almost all tissues.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (92%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of HEK-293 cells, using IFT20 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HEK-293 cells, using IFT20 antibody.)
IHC (Immunohistochemistry)
(AAA322841 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA322841 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-IFT20 antibody
Function: Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
Subunit Structure: Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts directly with IFT57 and KIF3B/Kinesin II subunit. Interacts with IFT88 (By similarity). Interacts with CEP83.
Subunit Structure: Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts directly with IFT57 and KIF3B/Kinesin II subunit. Interacts with IFT88 (By similarity). Interacts with CEP83.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 15-18 kDa
Predicted: 16 kDa
Predicted: 16 kDa
NCBI Official Full Name
intraflagellar transport protein 20 homolog isoform 1
NCBI Official Synonym Full Names
intraflagellar transport 20
NCBI Official Symbol
IFT20
NCBI Protein Information
intraflagellar transport protein 20 homolog
UniProt Protein Name
Intraflagellar transport protein 20 homolog
UniProt Gene Name
IFT20
UniProt Synonym Gene Names
hIFT20
Similar Products
Product Notes
The IFT20 ift20 (Catalog #AAA322841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFT20 Antibody reacts with Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's IFT20 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IFT20 ift20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFT20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.