WB (Western Blot)
(Western blot analysis IgA using Jurkat whole cell lysates)
Rabbit anti-Human, Rat IgA Polyclonal Antibody | anti-IGHA1 antibody
IgA Antibody
Gene Names
IGHA1; IgA1
Reactivity
Human, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IgA, Antibody; IgA Antibody; FLJ14473; FLJ35065; FLJ35500; FLJ36402; FLJ39698; FLJ40001; FLJ41548; FLJ41552; FLJ41789; FLJ43248; FLJ43594; FLJ44293; FLJ46028; FLJ46621; FLJ46724; FLJ46811; FLJ46824; FLJ90170; Ig alpha 1 chain C region; IgA1; IGHA1; immunoglobulin heavy constant alpha 1; MGC102857; A2m marker; Ig alpha 2 chain C region; IgA constant heavy chain 2; IGHA 2; IGHA2; IGHA2 protein; Immunoglobulin A2; Immunoglobulin Am2; immunoglobulin heavy constant alpha 2 (A2m marker); Immunoglobulin heavy constant alpha 2; anti-IGHA1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
IgA antibody detects endogenous levels of total IgA
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
353
Applicable Applications for anti-IGHA1 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human IgA
Subcellular Location
Secreted
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis IgA using Jurkat whole cell lysates)
WB (Western Blot)
(Western blot analysis IgA using Jurkat whole cell lysates)
IHC (Immunohistochemistry)
(AAA327522 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA327522 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-IGHA1 antibody
Function: Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed:2241915).
Subunit Structure: Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked (PubMed:20176268). Monomeric or polymeric (PubMed:2241915).
Post-translational Modifications: 3-Hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with alpha-1-microglobulin to form heterogeneous polycyclic chromophores including hydroxanthommatin. The chromophore reacts with accessible cysteines forming non-reducible thioether cross-links with Ig alpha-1 chain C region Cys-352. N-and O-glycosylated. N-glycan at Asn-144: Hex5HexNAc4.
Subunit Structure: Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked (PubMed:20176268). Monomeric or polymeric (PubMed:2241915).
Post-translational Modifications: 3-Hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with alpha-1-microglobulin to form heterogeneous polycyclic chromophores including hydroxanthommatin. The chromophore reacts with accessible cysteines forming non-reducible thioether cross-links with Ig alpha-1 chain C region Cys-352. N-and O-glycosylated. N-glycan at Asn-144: Hex5HexNAc4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed: 37 kDa
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
Immunoglobulin heavy constant alpha 1
NCBI Official Synonym Full Names
immunoglobulin heavy constant alpha 1
NCBI Official Symbol
IGHA1
NCBI Official Synonym Symbols
IgA1
UniProt Protein Name
Immunoglobulin heavy constant alpha 1
UniProt Gene Name
IGHA1
Similar Products
Product Notes
The IGHA1 igha1 (Catalog #AAA327522) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IgA Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IgA can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IGHA1 igha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.