Application Data
(Optical PropertiesIt is possible that the conjugate tag may bind in the paratope of the antibody, thereby limiting binding of the antibody to the antigen. Although this is unlikely, it could affect the ability of the antibody to bind to the antigen in various species and applications. As we cannot control the binding of the conjugate to the antibody, there is no way to confirm or guarantee the location of the antibody tag.)
Rabbit anti-Human; Mouse IGF-1 Polyclonal Antibody | anti-IGF-1 antibody
IGF-1 Antibody: ATTO 488
High photostability
Very hydrophilic
Excellent solubility in water
Vet little aggregation
New dye with net charge of -1
Application Data
(Optical PropertiesIt is possible that the conjugate tag may bind in the paratope of the antibody, thereby limiting binding of the antibody to the antigen. Although this is unlikely, it could affect the ability of the antibody to bind to the antigen in various species and applications. As we cannot control the binding of the conjugate to the antibody, there is no way to confirm or guarantee the location of the antibody tag.)
Application Data
(Optical PropertiesIt is possible that the conjugate tag may bind in the paratope of the antibody, thereby limiting binding of the antibody to the antigen. Although this is unlikely, it could affect the ability of the antibody to bind to the antigen in various species and applications. As we cannot control the binding of the conjugate to the antibody, there is no way to confirm or guarantee the location of the antibody tag.)
Application Data
(Chemical Structure)
Application Data
(Chemical Structure)
WB (Western Blot)
(Western blot analysis of Mouse Liver cell lysates showing detection of ~21.8 kDa IGF-1 protein using Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~21.8 kDa.)
WB (Western Blot)
(Western blot analysis of Mouse Liver cell lysates showing detection of ~21.8 kDa IGF-1 protein using Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~21.8 kDa.)
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IGF-1 Antibody. (D) Composite.)
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody (SPC-699) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IGF-1 Antibody. (D) Composite.)
NCBI and Uniprot Product Information
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Product Notes
The IGF-1 igf1 (Catalog #AAA253825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IGF-1 Antibody: ATTO 488 reacts with Human; Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IGF-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IGF-1 igf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGF-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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