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IHC (Immunohistochemistry)
(IGF1 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Goat anti-Human IGF1 Polyclonal Antibody | anti-IGF1 antibody
IGF1 Goat anti-Human Polyclonal (aa100-109) Antibody
Gene Names
IGF1; IGFI; IGF-I; IGF1A
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
IGF1, Antibody; IGF1 Goat anti-Human Polyclonal (aa100-109) Antibody; IHC-plus IGF1 Antibody (aa100-109); IGF1; IGF-IA; IGF-IB; IGFI; IBP1; IGF-I; Insulin-like growth factor 1; Insulin-like growth factor I; Insulin-like growth factor IA; Insulin-like growth factor IB; Mechano growth factor; Somatomedin-C; IGF1A; MGF; anti-IGF1 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Specificity
Human IGF1. This antibody is expected to recognize all reported isoforms (NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1).
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
TBS, pH7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-IGF1 antibody
WB (Western Blot), ELISA (Peptide ELISA), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence)
Target
Human IGF1
Immunogen
Peptide with sequence C-RSVRAQRHTD, from the internal region of the protein sequence according to NP_001104753.1NP_001104754.1NP_001104755.1NP_000609.1.
Conjugation
Unconjugated
Epitope
aa100-109
Family
Hormone
Preparation and Storage
Aliquot and store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemistry)
(IGF1 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(IGF1 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(IGF1 Antibody-IGF1 antibody (0.5 ug/ml) staining of Human Uterus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(IGF1 Antibody-IGF1 antibody (0.5 ug/ml) staining of Human Uterus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(IGF1 Antibody-IGF1 antibody (0.5ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
(IGF1 Antibody-IGF1 antibody (0.5ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
ICC (Immunocytochemistry)
(IGF1 Antibody-IGF1 Antibody Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
ICC (Immunocytochemistry)
(IGF1 Antibody-IGF1 Antibody Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
FCM/FACS (Flow Cytometry)
(IGF1 Antibody-IGF1 Antibody Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(IGF1 Antibody-IGF1 Antibody Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-IGF1 antibody
IGF1 antibody is an unconjugated goat polyclonal antibody to IGF1 (aa100-109) from human. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,762 Da
NCBI Official Full Name
insulin-like growth factor I isoform 4 preproprotein
NCBI Official Synonym Full Names
insulin-like growth factor 1 (somatomedin C)
NCBI Official Symbol
IGF1
NCBI Official Synonym Symbols
IGFI; IGF-I; IGF1A
NCBI Protein Information
insulin-like growth factor I; IGF-IA; IGF-IB; MGF; insulin-like growth factor IA; insulin-like growth factor IB; mechano growth factor; somatomedin-C
UniProt Protein Name
Insulin-like growth factor I
UniProt Gene Name
IGF1
UniProt Synonym Gene Names
IBP1; IGF-I; MGF
UniProt Entry Name
IGF1_HUMAN
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Product Notes
The IGF1 igf1 (Catalog #AAA162586) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The IGF1 Goat anti-Human Polyclonal (aa100-109) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IGF1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA (Peptide ELISA), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the IGF1 igf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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