Rabbit anti-Rhesus IGFBP2 Polyclonal Antibody | anti-IGFBP2 antibody

Anti-IGFBP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA257354

• Reactivity: Rhesus
• Applications: Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: IGFBP2, Antibody; Anti-IGFBP2 Antibody, Rabbit Polyclonal; IGFBP-2/IGFBP2 Antibody, Rabbit PAb, Antigen Affinity Purified; insulin-like growth factor binding protein 2, 36kDa; anti-IGFBP2 antibody

• Host: Rabbit
• Reactivity: Rhesus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rhesus IGFBP-2/IGFBP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IGFBP2 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Rhesus IGFBP-2/IGFBP2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rhesus IGFBP-2/IGFBP2. IGFBP-2/IGFBP2 specific IgG was purified by Rhesus IGFBP-2/IGFBP2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-IGFBP rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: A549 Whole Cell LysateLane C: SH-SY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa)

IP (Immunoprecipitation)

(cyno IGFBP2 was immunoprecipitated using:Lane A:0.5 mg SH-SY5Y Whole Cell Lysate2 uL anti-cyno IGFBP2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-cyno IGFBP2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa)

IHC (Immunohiostchemistry)

(Immunochemical staining of Rhesus IGFBP2 in Rhesus heart with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry)

(Immunochemical staining of Rhesus IGFBP2 in Rhesus kidney with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-IGFBP2 antibody

IGFBP-2, also known as IGFBP2, is an insulin-like growth factor-binding protein (IGFBP). IGFBPs prolong the half-life of the IGFs, control bioavailability, activity, and distribution of insulin-like growth factor (IGF) through high-affinity IGFBP/IGF complexes. Six high-affinity IGF-binding proteins (IGFBP-1 to -6) have been identified. The six IGFBPs are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP-2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to the potential involvement of IGFBP-2 in tumorigenesis. It contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. It inhibits IGF-mediated growth and developmental rates.

References

Han VK, et al. (1996) The expression of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) genes in the human placenta and membranes: evidence for IGF-IGFBP interactions at the feto-maternal interface. J Clin Endocrinol Metab. 81(7): 2680-93.Binkert C, et al. (1989) Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein (IGFBP-2). EMBO J. 8(9):2497-502.Wolf E, et al. (2000) Effects of IGFBP-2 overexpression in vitro and in vivo. Pediatr Nephrol. 14 (7):572-8.

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