WB (Western Blot)
(Detection of human PCNA by western blot with HRP-conjugated Goat anti-Mouse IgG Heavy and Light Chain Antibody. Samples: Whole cell lysate (50 ug) from Hela, Jurkat, K-562, A549, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-PCNA Monoclonal Antibody [PC10] used for WB at 1:1000. Secondary: HRP-conjugated Goat anti-Mouse IgG Heavy and Light Chain Antibody (AAA213712). Detection: Chemiluminescence with an exposure time of 3 seconds.)
IgG Heavy and Light Chain Secondary Antibody | IgG secondary antibody
Goat anti-Mouse IgG Heavy and Light Chain Antibody HRP Conjugated
Reactivity
Mouse
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
IgG Heavy and Light Chain, Secondary Antibody; Goat anti-Mouse IgG Heavy and Light Chain Antibody HRP Conjugated; Mouse IgG-Heavy and Light Chain; IgG secondary antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Concentration
1mg/ml (varies by lot)
Applicable Applications for IgG secondary antibody
ELISA, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Conjugation
HRP
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PCNA by western blot with HRP-conjugated Goat anti-Mouse IgG Heavy and Light Chain Antibody. Samples: Whole cell lysate (50 ug) from Hela, Jurkat, K-562, A549, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-PCNA Monoclonal Antibody [PC10] used for WB at 1:1000. Secondary: HRP-conjugated Goat anti-Mouse IgG Heavy and Light Chain Antibody (AAA213712). Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human PCNA by western blot with HRP-conjugated Goat anti-Mouse IgG Heavy and Light Chain Antibody. Samples: Whole cell lysate (50 ug) from Hela, Jurkat, K-562, A549, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-PCNA Monoclonal Antibody [PC10] used for WB at 1:1000. Secondary: HRP-conjugated Goat anti-Mouse IgG Heavy and Light Chain Antibody (AAA213712). Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human p53 by immunoperoxidase. Samples: FFPE serial sections of human stomach carcinoma. Primary Antibody: mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: goat anti-mouse IgG-heavy and light chain Antibody HRP Conjugated (AAA213712) used at a dilution of 1:200 (5ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human p53 by immunoperoxidase. Samples: FFPE serial sections of human stomach carcinoma. Primary Antibody: mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: goat anti-mouse IgG-heavy and light chain Antibody HRP Conjugated (AAA213712) used at a dilution of 1:200 (5ug/ml). Detection: DAB)
Related Product Information for IgG secondary antibody
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Product Categories/Family for IgG secondary antibody
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Product Notes
The IgG (Catalog #AAA213712) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Mouse IgG Heavy and Light Chain Antibody HRP Conjugated reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IgG Heavy and Light Chain can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG Heavy and Light Chain, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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